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Investigation of Ammonia Transport Pathways in the Anal Papillae of Larval Aedes Aegypti Mosquito

Investigation of Ammonia Transport Pathways in the Anal Papillae of Larval Aedes Aegypti Mosquito

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Title: Investigation of Ammonia Transport Pathways in the Anal Papillae of Larval Aedes Aegypti Mosquito
Author: Ionescu, Razvan Adrian
Abstract: The anal papillae of Aedes aegypti larvae, are important sites of ammonia/ammonium excretion. They express three putative ammonia (NH3/NH4+) transporters, Rhesus proteins AeRh50-1, AeRh50-2 and AeAmt. Ammonium concentration gradients were measured adjacent to the anal papillae using scanning ion-selective electrode technique (SIET). Results suggest that the ionomotive pumps V-type H+ ATPase (VA) and Na+/K+ ATPase (NKA) as well as cation / H+ exchanger, isoform 3 (NHE3) participate in ammonia excretion at the anal papillae. VA is involved in ammonia trapping at the exterior unstirred layer of the anal papillae, while basal NKA and apical NHE3 facilitate the transport of NH4+. Quantitative RT-PCR analysis revealed differential abundances of AeRh-2 and Amt in the anal papillae relative to the abundance of AeRh50-1. In presence of HEA both Rhs were down regulated but Amt was up regulated. It is the conclusion of this study that these transporters work in consortium to regulate ammonia.
Subject: Physiology
Animal sciences
Biology
Keywords: Aedes aegypti
larva
Mosquito
Vector
Yellow fever
Dengue
Chikunganya
Anal papilla
Rhesus glycoprotein
SIET
Pharmacology
Ion flux
Transport
NHE
NHE3
NKA
V-ATPase
K-channel
Ammonia
Ammonium
NH3
NH4+
qPCR
HEA
Type: Electronic Thesis or Dissertation
Rights: Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
URI: http://hdl.handle.net/10315/29920
Supervisor: Donini, Andrew
Degree: MSc - Master of Science
Program: Biology
Exam date: 2014-09-23
Publish on: 2015-08-28

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