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Investigation of Ammonia Transport Pathways in the Anal Papillae of Larval Aedes Aegypti Mosquito

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dc.contributor.advisorDonini, Andrew
dc.creatorIonescu, Razvan Adrian
dc.date.accessioned2015-08-28T15:02:36Z
dc.date.available2015-08-28T15:02:36Z
dc.date.copyright2014-09-23
dc.date.issued2015-08-28
dc.date.updated2015-08-28T15:02:36Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe anal papillae of Aedes aegypti larvae, are important sites of ammonia/ammonium excretion. They express three putative ammonia (NH3/NH4+) transporters, Rhesus proteins AeRh50-1, AeRh50-2 and AeAmt. Ammonium concentration gradients were measured adjacent to the anal papillae using scanning ion-selective electrode technique (SIET). Results suggest that the ionomotive pumps V-type H+ ATPase (VA) and Na+/K+ ATPase (NKA) as well as cation / H+ exchanger, isoform 3 (NHE3) participate in ammonia excretion at the anal papillae. VA is involved in ammonia trapping at the exterior unstirred layer of the anal papillae, while basal NKA and apical NHE3 facilitate the transport of NH4+. Quantitative RT-PCR analysis revealed differential abundances of AeRh-2 and Amt in the anal papillae relative to the abundance of AeRh50-1. In presence of HEA both Rhs were down regulated but Amt was up regulated. It is the conclusion of this study that these transporters work in consortium to regulate ammonia.
dc.identifier.urihttp://hdl.handle.net/10315/29920
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectPhysiology
dc.subjectAnimal sciences
dc.subjectBiology
dc.subject.keywordsAedes aegypti
dc.subject.keywordslarva
dc.subject.keywordsMosquito
dc.subject.keywordsVector
dc.subject.keywordsYellow fever
dc.subject.keywordsDengue
dc.subject.keywordsChikunganya
dc.subject.keywordsAnal papilla
dc.subject.keywordsRhesus glycoprotein
dc.subject.keywordsSIET
dc.subject.keywordsPharmacology
dc.subject.keywordsIon flux
dc.subject.keywordsTransport
dc.subject.keywordsNHE
dc.subject.keywordsNHE3
dc.subject.keywordsNKA
dc.subject.keywordsV-ATPase
dc.subject.keywordsK-channel
dc.subject.keywordsAmmonia
dc.subject.keywordsAmmonium
dc.subject.keywordsNH3
dc.subject.keywordsNH4+
dc.subject.keywordsqPCR
dc.subject.keywordsHEA
dc.titleInvestigation of Ammonia Transport Pathways in the Anal Papillae of Larval Aedes Aegypti Mosquito
dc.typeElectronic Thesis or Dissertationen_US

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