The effects of chronic muscle use and disuse on cardiolipin metabolism

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dc.contributor.advisorHood, David
dc.creatorOstojic, Olga
dc.date.accessioned2016-09-13T13:14:19Z
dc.date.available2016-09-13T13:14:19Z
dc.date.copyright2012-10
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractMitochondrial membranes have a diverse structure composed of numerous phospholipids, including cardiolipin (CL). Several processes are required to synthesize· and remodel CL. We previously demonstrated that CL content increases with chronic muscle use, and decreases with denervation. To investigate the mechanisms underlying these effects, we measured the mRNA expression of CL metabolism enzymes, including 1) CL synthesis enzymes Cardiolipin synthase (CLS) and CTP:PA-cytidylyltransferase-1 (CDS-1 ); 2) remodelling enzymes Tafazzin (Taz) and Acyl-CoA:lysocardiolipin acyltransferase-1 (ALCAT1); and 3) outer membrane CL enzymes, Mitochondrial phospholipase D (MitoPLD) and phospholipid scramblase 3 (Plscr3). We found that the transcriptional coactivator PGC-1? regulates the mRNA levels of CDS-1 and ALCAT1, thus playing a role in both CL synthesis and remodelling. We found that all three enzyme categories were altered by our conditions, but unaffected by age, suggesting that chronic muscle use and disuse have divergent influences on the expression of mRNAs encoding enzymes involved in CL metabolism.
dc.identifier.urihttp://hdl.handle.net/10315/31956
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subject.keywordsCardiolipin metabolism
dc.subject.keywordsChronic muscle use
dc.subject.keywordsCL
dc.subject.keywordsCardiolipin synthase
dc.subject.keywordsCLS
dc.subject.keywordsCTP:PA-cytidylyltransferase-1
dc.subject.keywordsCDS-1
dc.titleThe effects of chronic muscle use and disuse on cardiolipin metabolism
dc.typeElectronic Thesis or Dissertation

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