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Studies on the Function of the PRD-1 Gene in Neurospora Crassa

Studies on the Function of the PRD-1 Gene in Neurospora Crassa

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Title: Studies on the Function of the PRD-1 Gene in Neurospora Crassa
Author: Wu, Di
Abstract: In Neurospora crassa, conidiation is controlled by the circadian clock which consists of more than one oscillator. The frequency (frq) gene is a key component in the FRQ oscillator. The prd-1 mutant was found to lengthen the period of the circadian rhythm and disrupts the FRQ-less rhythm, but how this mutant affects the oscillators was unclear. This thesis aims to study the function of the prd-1 gene. Building on previous research, knocking out prd-1 is lethal; strains expressing PRD-1-FLAG were constructed; prd-1 expression showed non- circadian rhythmicity; co-IP and MS analysis discovered ribosomal proteins bind to PRD-1; the prd-1 mutant responds to amino acids differently in growth; and the deletion of the C-terminus of prd-1 was found to be lethal. The results of these various experiments outlined that the prd-1 gene may affect the circadian rhythm via nutrient sensing pathways especially the TOR pathway.
Subject: Molecular biology
Keywords: Neurospora crassa
Gene function
Circadian rhythm
Prd-1
Gene engineering
Nutrient sensing pathway
TOR pathway
Type: Electronic Thesis or Dissertation
Rights: Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
URI: http://hdl.handle.net/10315/35487
Supervisor: Lakin-Thomas, Patricia
Degree: MSc - Master of Science
Program: Biology
Exam date: 2018-06-13
Publish on: 2018-11-21

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