Studies on the Function of the PRD-1 Gene in Neurospora Crassa

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dc.contributor.advisorLakin-Thomas, Patricia
dc.creatorWu, Di
dc.date.accessioned2018-11-21T13:42:05Z
dc.date.available2018-11-21T13:42:05Z
dc.date.copyright2018-06-13
dc.date.issued2018-11-21
dc.date.updated2018-11-21T13:42:05Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractIn Neurospora crassa, conidiation is controlled by the circadian clock which consists of more than one oscillator. The frequency (frq) gene is a key component in the FRQ oscillator. The prd-1 mutant was found to lengthen the period of the circadian rhythm and disrupts the FRQ-less rhythm, but how this mutant affects the oscillators was unclear. This thesis aims to study the function of the prd-1 gene. Building on previous research, knocking out prd-1 is lethal; strains expressing PRD-1-FLAG were constructed; prd-1 expression showed non- circadian rhythmicity; co-IP and MS analysis discovered ribosomal proteins bind to PRD-1; the prd-1 mutant responds to amino acids differently in growth; and the deletion of the C-terminus of prd-1 was found to be lethal. The results of these various experiments outlined that the prd-1 gene may affect the circadian rhythm via nutrient sensing pathways especially the TOR pathway.
dc.identifier.urihttp://hdl.handle.net/10315/35487
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subject.keywordsNeurospora crassa
dc.subject.keywordsGene function
dc.subject.keywordsCircadian rhythm
dc.subject.keywordsPrd-1
dc.subject.keywordsGene engineering
dc.subject.keywordsNutrient sensing pathway
dc.subject.keywordsTOR pathway
dc.titleStudies on the Function of the PRD-1 Gene in Neurospora Crassa
dc.typeElectronic Thesis or Dissertation

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