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Distribution and Activity of CAPA-like Peptides in the Black-legged Tick, Ixodes scapularis

Distribution and Activity of CAPA-like Peptides in the Black-legged Tick, Ixodes scapularis

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Title: Distribution and Activity of CAPA-like Peptides in the Black-legged Tick, Ixodes scapularis
Author: Uyuklu, Ali
Abstract: Ixodes scapularis is a chief vector for a range of diseases. Due to their obligate blood feeding strategy, it is crucial for I. scapularis to maintain ionic and osmotic homeostasis. In insects, CAPA peptides act as either diuretic or anti-diuretic hormones controlling the movement of excess ions, water, and metabolic wastes secreted in the primary urine. Immunohistochemical techniques were used to localize CAPA-like peptides to the synganglion, midgut, reproductive tissues, and hindgut. Given the presence of CAPA-like immunoreactivity in the hindgut, in vitro bioassays were conducted. A significant increase in the frequency of hindgut contractions were observed when exposed to 1 M IxoscCAPA-PVK. RT-PCR techniques, however, solely localized the CAPA transcript to the tick synganglion. The immunoreactivity observed in the peripheral tissues, including the midgut, are indicative of the RFamide-related peptide, sNPF, as the transcript coincides with the immunoreactivity, and ELISA results revealing affinity of the primary antibody (rabbit anti-RhoprCAPA-2).
Subject: Animal diseases
Keywords: Biology
Ixodes scapularis
Tick
Lyme disease
CAPA
sNPF
Animal physiology
Pathogens
Ionic and osmotic homeostasis
Malpighian tubules
Distribution
Function
Immunohistochemistry
RT-PCR
ELISA
Hindgut
Impedance contraction assays
Bioassays
Hindgut contraction
Fluid regulation
Disease
Vector
Diuretic
Anti-diuretic
Type: Electronic Thesis or Dissertation
Rights: Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
URI: http://hdl.handle.net/10315/34954
Supervisor: Paluzzi, Jean-Paul
Degree: MSc - Master of Science
Program: Biology
Exam date: 2017-12-01
Publish on: 2018-08-27

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