Distribution and Activity of CAPA-like Peptides in the Black-legged Tick, Ixodes scapularis

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dc.contributor.advisorPaluzzi, Jean-Paul
dc.creatorUyuklu, Ali
dc.date.accessioned2018-08-27T16:28:39Z
dc.date.available2018-08-27T16:28:39Z
dc.date.copyright2017-12-01
dc.date.issued2018-08-27
dc.date.updated2018-08-27T16:28:39Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractIxodes scapularis is a chief vector for a range of diseases. Due to their obligate blood feeding strategy, it is crucial for I. scapularis to maintain ionic and osmotic homeostasis. In insects, CAPA peptides act as either diuretic or anti-diuretic hormones controlling the movement of excess ions, water, and metabolic wastes secreted in the primary urine. Immunohistochemical techniques were used to localize CAPA-like peptides to the synganglion, midgut, reproductive tissues, and hindgut. Given the presence of CAPA-like immunoreactivity in the hindgut, in vitro bioassays were conducted. A significant increase in the frequency of hindgut contractions were observed when exposed to 1 M IxoscCAPA-PVK. RT-PCR techniques, however, solely localized the CAPA transcript to the tick synganglion. The immunoreactivity observed in the peripheral tissues, including the midgut, are indicative of the RFamide-related peptide, sNPF, as the transcript coincides with the immunoreactivity, and ELISA results revealing affinity of the primary antibody (rabbit anti-RhoprCAPA-2).
dc.identifier.urihttp://hdl.handle.net/10315/34954
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectAnimal diseases
dc.subject.keywordsBiology
dc.subject.keywordsIxodes scapularis
dc.subject.keywordsTick
dc.subject.keywordsLyme disease
dc.subject.keywordsCAPA
dc.subject.keywordssNPF
dc.subject.keywordsAnimal physiology
dc.subject.keywordsPathogens
dc.subject.keywordsIonic and osmotic homeostasis
dc.subject.keywordsMalpighian tubules
dc.subject.keywordsDistribution
dc.subject.keywordsFunction
dc.subject.keywordsImmunohistochemistry
dc.subject.keywordsRT-PCR
dc.subject.keywordsELISA
dc.subject.keywordsHindgut
dc.subject.keywordsImpedance contraction assays
dc.subject.keywordsBioassays
dc.subject.keywordsHindgut contraction
dc.subject.keywordsFluid regulation
dc.subject.keywordsDisease
dc.subject.keywordsVector
dc.subject.keywordsDiuretic
dc.subject.keywordsAnti-diuretic
dc.titleDistribution and Activity of CAPA-like Peptides in the Black-legged Tick, Ixodes scapularis
dc.typeElectronic Thesis or Dissertation

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