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Studying Magnesium Transport in the Common Goldfish (Carassius auratus) Using Molecular and Potentiometric Approaches: Role and Characterization of the Magnesium Transporter Solute Carrier 41a1 (SLC41a1)

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Date

2017-07-27

Authors

Ivaylov Kodzhahinchev, Vladimir

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This thesis examined how magnesium transporter solute carrier 41a1 (SLC41a1) is regulated at the transcriptional level in the stenohaline goldfish (Carassius auratus) in response to environmental (ion-poor water exposures and temperature variation) and dietary manipulations (feeding a low, regular and high magnesium content diet). SLC41a1 mRNA behaved contrary to my hypotheses that expression levels would be innversely proportional to magnesium availability in the diet and environment. This suggests SLC41a1 participates in magnesium secretion, rather than absorption as proposed in mammals. Finally, the scanning ion-selective electrode technique (SIET) was used as an alternative to radioisotope approaches in magnesium transport study. SIET found transport zonation along the gastrointestinal (GI) tract for magnesium, suggestive of mostly passive transport kinetics for magnesium at the 1st GI tract segment. Furthermore, a decrease in magnesium transport at the 1st and 8th GI tract segments was observed in response to 10-4M ouabain, but not 10-4M cobalt(III)hexamine-chloride.

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Biology

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