Studying Magnesium Transport in the Common Goldfish (Carassius auratus) Using Molecular and Potentiometric Approaches: Role and Characterization of the Magnesium Transporter Solute Carrier 41a1 (SLC41a1)

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dc.contributor.advisorBucking, Carol
dc.creatorIvaylov Kodzhahinchev, Vladimir
dc.date.accessioned2017-07-27T13:33:09Z
dc.date.available2017-07-27T13:33:09Z
dc.date.copyright2016-12-08
dc.date.issued2017-07-27
dc.date.updated2017-07-27T13:33:09Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThis thesis examined how magnesium transporter solute carrier 41a1 (SLC41a1) is regulated at the transcriptional level in the stenohaline goldfish (Carassius auratus) in response to environmental (ion-poor water exposures and temperature variation) and dietary manipulations (feeding a low, regular and high magnesium content diet). SLC41a1 mRNA behaved contrary to my hypotheses that expression levels would be innversely proportional to magnesium availability in the diet and environment. This suggests SLC41a1 participates in magnesium secretion, rather than absorption as proposed in mammals. Finally, the scanning ion-selective electrode technique (SIET) was used as an alternative to radioisotope approaches in magnesium transport study. SIET found transport zonation along the gastrointestinal (GI) tract for magnesium, suggestive of mostly passive transport kinetics for magnesium at the 1st GI tract segment. Furthermore, a decrease in magnesium transport at the 1st and 8th GI tract segments was observed in response to 10-4M ouabain, but not 10-4M cobalt(III)hexamine-chloride.
dc.identifier.urihttp://hdl.handle.net/10315/33506
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsMagnesium transport
dc.subject.keywordsGoldfish
dc.subject.keywordsCarassius auratus
dc.subject.keywordsSIET
dc.subject.keywordsqPCR
dc.subject.keywordsOuabain
dc.subject.keywordscobalt(3)hexaamine-chloride
dc.subject.keywordsSLC41a1
dc.subject.keywordsTRPM6
dc.subject.keywordsTRPM7
dc.subject.keywordsmRNA
dc.subject.keywordsDiet
dc.subject.keywordsTemperature
dc.subject.keywordsZonation
dc.subject.keywordsGill
dc.subject.keywordsGI tract
dc.subject.keywordsTransport kinetics
dc.subject.keywordsGastrointestinal tract
dc.titleStudying Magnesium Transport in the Common Goldfish (Carassius auratus) Using Molecular and Potentiometric Approaches: Role and Characterization of the Magnesium Transporter Solute Carrier 41a1 (SLC41a1)
dc.typeElectronic Thesis or Dissertation

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