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Front-End Methods For Enhancing the Analytical Power of Mass Spectrometry
(2016-09-20)
The analytical power and versatility of mass spectrometry can be enhanced by adding front-end devices, which provide additional functionality before, during or immediately after ElectroSpray Ionization (ESI). Such devices ...
Part I: Crystallization of A Type IV Pilin from Pseudomonas Aeruginosa. Part II: Characterization of a Peptidyl-Prolyl-Cis,Trans-Isomerase Through X-Ray Crystallography
(2018-05-28)
Within a host, pathogenic bacteria employ several mechanisms that enhance their survival and motility. These mechanisms include their ability to adhere, replicate (in order to avoid eradication), and secretion of virulent ...
The Cocaine-Binding Aptamer: A Thermodynamic and Structural Overview of Specific and Non-Specific Binding Interactions
(2016-09-20)
Isothermal Titration Calorimetry (ITC) studies were conducted wherein variances in the heat capacity of binding (Cp) among variants of the cocaine-binding aptamer were suggestive of two distinct binding mechanisms. Aptamer ...
Studies of Protein Function by Liquid Chromatography-Mass Spectrometry
(2015-01-26)
Complete genomes of many organisms have been recorded using high throughput nucleic acid sequencing; however the intricate functions of the gene products remain largely undiscovered. As the most prominent technology for ...
Biomedical Characterization of the WalKR and the LytSR Two Component System From Staphylococcus Aureus
(2015-08-28)
Staphylococcus aureus is a major gram-positive pathogen, because of its remarkable capacity to develop resistance against different antimicrobial agents. It has been shown that in S. aureus two component systems (TCS) ...
Structural Studies of the Receiver Domain of LytR from Staphylococcus Aureus and Interaction Studies of TraW and TrbC from the F Plasmid of Escherichia Coli
(2015-01-26)
The emergence of the multi-drug resistant bacteria and the evolutionary spread of the antibiotic resistance genes have become a great concern to human health. Bacteria use various systems in combination or alone in response ...
Characterizing Protein Dynamics of Protein-Ligand Interactions by Hydrogen-Deuterium Exchange Mass Spectrometry
(2015-01-26)
The study of protein-ligand and protein-protein interactions is of paramount importance to the understanding of their biological function. Whereas this area of research has been largely dominated by conventional structural ...
Characterization of Function and Role of FMTA in Staphylococcus Aureus
(2015-08-28)
Staphylococcus aureus is one of the major reasons for infectious mortality and morbidity. Due to extensive use of antibiotics, S. aureus has acquired resistance to almost all antibiotics. FmtA is one of the additional ...
Obtaining Structural Insights on Bacterial Protein Complexes Using Time-Resolved Hydrogen-Deuterium Exchange Mass Spectrometry
(2015-12-16)
Persistent infections by Pseudomonas aeruginosa are initiated by interaction of a type IV pilus (T4P) with receptors on the mucosal cells of susceptible hosts. Here, we examine the structural changes occurring between the ...
Measuring the Dynamics and Kinetics of OXA-58, a Class D Abeta-Lactamase from Acinetobacter Baumannii
(2015-12-16)
OXA-58 is a β-lactamase enzyme that bacteria Acinetobacter baumannii employ as their chief defense mechanism against β-lactam antibiotic treatment. The catalytic activity of OXA-58 and its mutants was monitored as they ...