Integrated Two-Dimensional/Three-Dimensional Collagen Scaffolds with Embedded Channels

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dc.contributor.advisorSachlos, Terry
dc.contributor.advisorRezai, Pouya
dc.contributor.authorFakhri, Neda
dc.date.accessioned2023-12-08T14:21:09Z
dc.date.available2023-12-08T14:21:09Z
dc.date.issued2023-12-08
dc.date.updated2023-12-08T14:21:09Z
dc.degree.disciplineMechanical Engineering
dc.degree.levelMaster's
dc.degree.nameMASc - Master of Applied Science
dc.description.abstractWe developed a two-step method for simple integration of basement membrane (BM) in a 3D porous scaffold (i.e., interstitial matrix (IM) ) embedded with channels wherein the 2D BM analog was fabricated by casting collagen on a positive master mold and slowly air-dried at room temperature to replicate the microchannel feature on the mold. Then, collagen slurry was poured over the 2D collagen film while it is still supported by the mold and the whole assembly is freeze-dried. The contact printing method was used to enclose channels with another non-patterned integrated collagen scaffold. The channels with and without the 2D BM were tested for leakage. The results showed no leakage when 2D collagen films were included in the scaffolds, even at flow rates as high as 25 ml/min. The present ECM model can be used in modeling tissues containing large vessels where the membrane restricts the permeation of fluid.
dc.identifier.urihttps://hdl.handle.net/10315/41590
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMechanical engineering
dc.subject.keywordsTissue engineering
dc.subject.keywordsExtracellular matrix
dc.subject.keywordsBasement membrane
dc.subject.keywordsInterstitial matrix
dc.subject.keywordsCollagen-based scaffold
dc.subject.keywordsFreeze-drying
dc.subject.keywordsVascular network
dc.subject.keywordsLumen
dc.subject.keywordsMicrochannel fabrication
dc.subject.keywordsMicrofabrication
dc.subject.keywordsMicropatterning
dc.subject.keywordsMicrofluidics
dc.titleIntegrated Two-Dimensional/Three-Dimensional Collagen Scaffolds with Embedded Channels
dc.typeElectronic Thesis or Dissertation

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