Integrated Two-Dimensional/Three-Dimensional Collagen Scaffolds with Embedded Channels

We developed a two-step method for simple integration of basement membrane (BM) in a 3D porous scaffold (i.e., interstitial matrix (IM) ) embedded with channels wherein the 2D BM analog was fabricated by casting collagen on a positive master mold and slowly air-dried at room temperature to replicate the microchannel feature on the mold. Then, collagen slurry was poured over the 2D collagen film while it is still supported by the mold and the whole assembly is freeze-dried. The contact printing method was used to enclose channels with another non-patterned integrated collagen scaffold. The channels with and without the 2D BM were tested for leakage. The results showed no leakage when 2D collagen films were included in the scaffolds, even at flow rates as high as 25 ml/min. The present ECM model can be used in modeling tissues containing large vessels where the membrane restricts the permeation of fluid.