Aquaporin AaAQP2 protein abundance in the anal papillae of Aedes aegypti is modified by salinity

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dc.contributor.advisorDonini, Andrew
dc.contributor.authorGuardian, Elia Edith Grieco
dc.date.accessioned2021-11-15T15:17:41Z
dc.date.available2021-11-15T15:17:41Z
dc.date.copyright2021-05
dc.date.issued2021-11-15
dc.date.updated2021-11-15T15:17:41Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe Aedes aegypti mosquito, a viral disease vector, can complete its life cycle in breeding sites that range in salinity from ion-poor to fresh and brackish water. Fluctuations in salinity provide different challenges to larval regulation of ion and water transport. Western blots of proposed water channel A. aegypti aquaporin 2 (AaAQP2) revealed this protein is found in the osmoregulatory organs, including gastric caecae, Malpighian tubules (MTs), hindgut, and anal papillae (AP) of larvae reared in varying salinity. AaAQP2 immunolocalization in MTs shifted from apical to intracellular as salinity increased, and could imply AaAQP2 is trafficked from the cell membrane to potentially decrease MT water secretion. Protein abundance of the presumed AaAQP2 monomer in AP increased as salinity increased, however, larval survival increased when larvae with knockdown AaAQP2 in the AP were transferred to higher salinity. Results suggest AaAQP2 protein levels impact larval physiology when changes in environmental salinity occur.
dc.identifier.urihttp://hdl.handle.net/10315/38654
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectPhysiology
dc.subject.keywordsAquaporin
dc.subject.keywordsAquaporins
dc.subject.keywordsMosquito
dc.subject.keywordsMosquitos
dc.subject.keywordsMosquitoes
dc.subject.keywordsLarva
dc.subject.keywordsLarvae
dc.subject.keywordsLarval
dc.subject.keywordsAquatic
dc.subject.keywordsAQP
dc.subject.keywordsAQPs
dc.subject.keywordsAQP2
dc.subject.keywordsAaAQP2
dc.subject.keywordsAaAQP
dc.subject.keywordsAaAQPs
dc.subject.keywordsAedes aegypti
dc.subject.keywordsA. aegypti
dc.subject.keywordsAedes
dc.subject.keywordsOsmoregulation
dc.subject.keywordsAnal papilla
dc.subject.keywordsAnal papillae
dc.subject.keywordsMalpighian tubule
dc.subject.keywordsGastric caeca
dc.subject.keywordsHindgut
dc.subject.keywordsOsmoregulatory organs
dc.subject.keywordsEpithelia
dc.subject.keywordsImmunohistochemistry
dc.subject.keywordsImmunolocalization
dc.titleAquaporin AaAQP2 protein abundance in the anal papillae of Aedes aegypti is modified by salinity
dc.typeElectronic Thesis or Dissertation

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