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The Heterodimeric Glycoprotein Hormone, GPA2/GPB5, Regulates Ion Transport across the Hindgut of the Adult Mosquito, Aedes aegypti

The Heterodimeric Glycoprotein Hormone, GPA2/GPB5, Regulates Ion Transport across the Hindgut of the Adult Mosquito, Aedes aegypti

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Title: The Heterodimeric Glycoprotein Hormone, GPA2/GPB5, Regulates Ion Transport across the Hindgut of the Adult Mosquito, Aedes aegypti
Author: Paluzzi, Jean-Paul
Vanderveken, Mark
O’Donnell, Michael J.
Abstract: A family of evolutionarily old hormones is the glycoprotein cysteine knot-forming eterodimers consisting of alpha- (GPA) and beta-subunits (GPB), which assemble by noncovalent bonds. In mammals, a common glycoprotein hormone alphasubunit
(GPA1) pairs with unique beta-subunits that establish receptor specificity, forming thyroid stimulating hormone (GPA1/TSHb) and the gonadotropins luteinizing hormone GPA1/LHb), follicle stimulating hormone (GPA1/FSHb), choriogonadotropin (GPA1/CGb). A novel glycoprotein heterodimer was identified in vertebrates by genome analysis,
called thyrostimulin, composed of two novel subunits, GPA2 and GPB5, and homologs occur in arthropods, nematodes and cnidarians, implying that this neurohormone system existed prior to the emergence of bilateral metazoans. In order to discern possible physiological roles of this hormonal signaling system in mosquitoes, we have isolated the glycoprotein hormone genes producing the alpha- and beta-subunits (AedaeGPA2 and AedaeGPB5) and assessed their temporal expression profiles in the yellow and dengue-fever vector, Aedes aegypti. We have also isolated a putative receptor for this novel mosquito hormone, AedaeLGR1, which contains features conserved with other glycoprotein leucine-rich repeating containing G protein-coupled receptors. AedaeLGR1 is expressed in tissues of the alimentary canal such as the midgut,
Malpighian tubules and hindgut, suggesting that this novel mosquito glycoprotein hormone may regulate ionic and osmotic balance. Focusing on the hindgut in adult stage A. aegypti, where AedaeLGR1 was highly enriched, we utilized the Scanning Ion-selective Electrode Technique (SIET) to determine if AedaeGPA2/GPB5 modulated cation transport across this epithelial tissue. Our results suggest that AedaeGPA2/GPB5 does indeed participate in ionic and osmotic balance, since it appears to inhibit natriuresis and promote kaliuresis. Taken together, our findings imply this hormone may play an
important role in ionic balance when levels of Na+ are limited and levels of K+ are in excess – such as during the digestion and assimilation of erythrocytes following vertebrate blood-feeding by females.
Sponsor: This research was supported through an NSERC postdoctoral fellowship to J.P.P. and an NSERC Discovery Grant and Discovery Accelerator Supplement to MJO. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publication was made possible by the York University Libraries' Open Access Author Fund.
Type: Article
Rights: Attribution 2.5 Canada
10.1371/journal.pone.0086386 http://www.plosone.org/
http://creativecommons.org/licenses/by/2.5/ca/
URI: http://hdl.handle.net/10315/27082
Published: PLoS ONE
Series: York University Libraries' Open Access Author Fund
Citation: Paluzzi J-P, Vanderveken M, O’Donnell MJ (2014) The Heterodimeric Glycoprotein Hormone, GPA2/GPB5, Regulates Ion Transport across the Hindgut of the Adult Mosquito, Aedes aegypti. PLoS ONE 9(1): e86386. doi:10.1371/journal.pone.0086386
Date: 2014-01-20

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Attribution 2.5 Canada Except where otherwise noted, this item's license is described as Attribution 2.5 Canada