Macromolecular Interactome of Tetrahymena CHD Family Chromatin Remodelers

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dc.contributor.advisorPearman, Ronald
dc.creatorDeRango-Adem, Eva Francesca
dc.date.accessioned2018-05-28T12:46:52Z
dc.date.available2018-05-28T12:46:52Z
dc.date.copyright2017-10-20
dc.date.issued2018-05-28
dc.date.updated2018-05-28T12:46:52Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractTetrahymena thermophila undergoes RNA-guided, DNA elimination during development. Bioinformatic and functional analyses on tChd family chromatin remodelers suggest the proteins are candidate DNA and RNA binding proteins. In vivo and in vitro functional analyses on tChd3 and tChd7 identified diverse macromolecular interactions throughout the lifecycle. Chd3 bound a novel protein named Miz1, which is a candidate E3 SUMO ligase. Chd3 and Miz1 co-localize to vegetatively repressed PDD1, and may regulate transcription via epigenetic SUMOylation of chromatin. Chd7 binds (un)modified H3K36, and highly transcribed genes during vegetative growth. This binding may regulate transcriptional turnover by Chd7-mediated reading of the acetyl/methyl status at H3K36. In vitro analyses suggest Chd7 binds poly(G) RNAs. G-rich sequences are transcribed during RNA-guided DNA elimination events, and Chd7 may be involved during this part of Tetrahymena development. These analyses provide a glimpse into how RNAs and epigenetics orchestrate chromatin dynamics, in complex eukaryotic regulatory mechanisms.
dc.identifier.urihttp://hdl.handle.net/10315/34494
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectCellular biology
dc.subject.keywordsRNA
dc.subject.keywordsChromatin
dc.subject.keywordsProtein Interactions
dc.subject.keywordsEpigenetics
dc.subject.keywordsChromatin Remodeling
dc.subject.keywordsTetrahymena
dc.subject.keywordsMicroscopy
dc.subject.keywordsMass Spectrometry
dc.subject.keywordsImmunoprecipitation
dc.subject.keywordsSUMOylation
dc.subject.keywordsMethylation
dc.subject.keywordsAcetylation
dc.subject.keywordsHistones
dc.subject.keywordslncRNAs
dc.subject.keywordssiRNAs
dc.subject.keywordsCHD
dc.subject.keywordsZinc Finger
dc.subject.keywordsPHD
dc.subject.keywordsBromodomain
dc.subject.keywordsChromodomain
dc.subject.keywordsMIZ-RING
dc.subject.keywordsATPase
dc.titleMacromolecular Interactome of Tetrahymena CHD Family Chromatin Remodelers
dc.typeElectronic Thesis or Dissertation

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