Exploring the role of mitochondria and muscle function in a pertussis toxin free experimental autoimmune myositis mouse model

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dc.contributor.advisorPerry, Christopher
dc.contributor.advisorAbdul-Sater, Ali
dc.contributor.authorGaribotti, Madison Claire
dc.date.accessioned2023-12-08T14:42:43Z
dc.date.available2023-12-08T14:42:43Z
dc.date.issued2023-12-08
dc.date.updated2023-12-08T14:42:43Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractMyositis is a rare autoimmune disorder characterized by skeletal muscle inflammation and weakness. Recently, mitochondrial stress has emerged as a possible disease contributor. To assess the relationship between mitochondrial stress and myopathy in myositis, evaluations of experimental models are required. Several models utilize an exotoxin, pertussis toxin(PT), coupled with foreign myosin injections. However, PT induces systemic inflammation impacting the ability to determine if the inflammatory response is consistent with myositis or a systemic response. Thus, the purpose of this thesis is to establish a modified myositis model whereby PT is excluded to exemplify the human condition, and to explore mitochondrial stress and myopathy. This modified model demonstrated no muscle atrophy occurred, but both the diaphragm and tibialis anterior(TA) displayed indices of mitochondrial stress. Additionally, the TA exhibited weakness that recovered over time. Suggesting the modified myositis model may induce a mild phenotype, highlighting the need for more robust model development.
dc.identifier.urihttps://hdl.handle.net/10315/41740
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectPhysiology
dc.subjectKinesiology
dc.subjectMolecular biology
dc.subject.keywordsMyositis
dc.subject.keywordsMuscle health
dc.subject.keywordsMitochondrial bioenergetics
dc.subject.keywordsPhysiology
dc.subject.keywordsImmunology
dc.subject.keywordsMetabolism
dc.titleExploring the role of mitochondria and muscle function in a pertussis toxin free experimental autoimmune myositis mouse model
dc.typeElectronic Thesis or Dissertation

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