Identification of Novel Substrates and Binding Partners of Speckle-Type POZ Protein Adapter from Cullin3-Ring E3 Ubiquitin Ligase Complex

Simple item page
dc.contributor.advisorSaridakis, Vivian
dc.contributor.authorLiu, Helen
dc.date.accessioned2022-03-03T13:54:39Z
dc.date.available2022-03-03T13:54:39Z
dc.date.copyright2019-12
dc.date.issued2022-03-03
dc.date.updated2022-03-03T13:54:38Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractSpeckle type POZ protein (SPOP) is a ubiquitin E3 ligase protein adapter found to be highly associated with prostate cancer. SPOP prostate cancer mutations were identified to be located within the Meprin and TRAF- C homology (MATH) domain of the protein, which is responsible for substrate recognition and interaction. This study focuses on identifying novel substrates/ binding partners of SPOP and characterizing their involvement in cancer initiation and progression. Due to the high sequence and structural similarity to the MATH domain of Ubiquitin- Specific Protease 7 (USP7) it is possible that there are many overlapping substrates/interacting partners between these two proteins. Eleven novel cellular and viral proteins that interact with SPOP were identified. The cellular proteins were shown to be destabilized upon SPOP over expression. Prostate cancer SPOP mutants showed loss of interaction with their substrates. These novel SPOP substrates/ binding partners bring insight into its involvement in cellular pathways and cancer development.
dc.identifier.urihttp://hdl.handle.net/10315/39037
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subject.keywordsSPOP
dc.subject.keywordsSpeckle type POZ protein
dc.subject.keywordsProstate cancer
dc.titleIdentification of Novel Substrates and Binding Partners of Speckle-Type POZ Protein Adapter from Cullin3-Ring E3 Ubiquitin Ligase Complex
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Liu_Helen_2019_Masters.pdf
Size:
2.2 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.87 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.39 KB
Format:
Plain Text
Description:
Download

Collections

Biology

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.