Identifying the Ecological Interactions of Invasive Lymantria Dispar Dispar and Agrilus Planipennis Using Metabarcoding Techniques

Simple item page
dc.contributor.advisorClare, Elizabeth
dc.contributor.authorBorsato, Nicole Dorothy
dc.date.accessioned2025-04-10T10:39:14Z
dc.date.available2025-04-10T10:39:14Z
dc.date.copyright2024-08-07
dc.date.issued2025-04-10
dc.date.updated2025-04-10T10:39:14Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe objectives of this thesis were to develop a standardized metabarcoding protocol to identify ecological interactions between insects and other taxa, as well as determine the impact of ecological correlates on the detection of these interactions. In Chapter 2, I evaluate target loci for the identification of Lymantria dispar dispar (spongy moth) and Agrilus planipennis (emerald ash borer) ecological interactions and develop a standardized protocol for the analysis of these interactions. I demonstrate that my metabarcoding protocol can be used to identify a wide diversity of interactions, including those with animals, plants, bacteria, and fungi. In Chapter 3, I assess how ecological factors (e.g., life stage, collection location, etc.) influence the presence or absence of ecological interactions in emerald ash borers using random forest models. I found that interactions were more likely to be detected in pupal specimens and in samples collected earlier in the year.
dc.identifier.urihttps://hdl.handle.net/10315/42725
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subject.keywordsMetabarcoding
dc.subject.keywordsEmerald ash borers
dc.subject.keywordsSpongy moths
dc.subject.keywordsAgrilus planipennis
dc.subject.keywordsLymantria dispar dispar
dc.subject.keywordsRandom forest
dc.subject.keywordsSymbiome
dc.subject.keywordsHolobiont
dc.subject.keywordsEcological interactions
dc.subject.keywordsrbcL
dc.subject.keywordsCOI
dc.subject.keywords16S
dc.subject.keywords18S
dc.subject.keywordsITS
dc.subject.keywordsInteractions
dc.subject.keywordsEnvironmental DNA
dc.subject.keywordseDNA
dc.subject.keywordsHigh‐throughput sequencing
dc.subject.keywordsDNA barcoding
dc.subject.keywordsInsect
dc.subject.keywordsInvasive species
dc.subject.keywordsBiological controls
dc.titleIdentifying the Ecological Interactions of Invasive Lymantria Dispar Dispar and Agrilus Planipennis Using Metabarcoding Techniques
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Nicole_Dorothy_Borsato_2024_MSc.pdf
Size:
1.96 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.87 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.39 KB
Format:
Plain Text
Description:
Download

Collections

Biology

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.