Optical Detection of Azithromycin in Water and Urine Using Molecularly Imprinted Polymer-Microfluidics Integration

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dc.contributor.advisorBrar, Satinder Kaur
dc.contributor.advisorRezai, Pouya
dc.contributor.authorNoha Mohamed Hosny Hasaneen
dc.date.accessioned2024-11-07T11:17:33Z
dc.date.available2024-11-07T11:17:33Z
dc.date.copyright2024-09-06
dc.date.issued2024-11-07
dc.date.updated2024-11-07T11:17:32Z
dc.degree.disciplineCivil Engineering
dc.degree.levelMaster's
dc.degree.nameMASc - Master of Applied Science
dc.description.abstractAzithromycin (AZM) is one of the most used antibiotics worldwide. Monitoring its concentration in urine and water is essential to control the spread of antibiotic-resistant bacteria. Two new methods for AZM analysis in artificial urine were developed using fluorescence-based microscopic and spectrophotometric techniques after forming an ion-pair complex with fluorescein isothiocyanate. The fluorescence method showed higher sensitivity and precision, with a linear range of 0-31.25 µg/mL, LOD of 0.41 µg/mL, and LOQ of 1.23 µg/mL, compared to the spectrophotometric one. Moreover, it showed a narrower recovery range of AZM from artificial urine samples, indicating higher precision in complex matrices compared to the spectrophotometric one. For enhanced selectivity and portability, a study on integrating molecularly imprinted polymer (MIP) as synthetic receptor inside a microfluidic device with the fluorescence-based detection method was performed. Despite the challenges with specificity, the study constitutes a promising detailed investigation where both extraction and detection of AZM can be done on the same platform using optical techniques. This approach can be potentially extended to enable multiplexing, where a microfluidic device with multiple channels, each containing an MIP specific for a different target compound can be used.
dc.identifier.urihttps://hdl.handle.net/10315/42505
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectCivil engineering
dc.subjectMechanical engineering
dc.subjectAnalytical chemistry
dc.subject.keywordsAntibiotic detection
dc.subject.keywordsOptical sensors
dc.subject.keywordsAntimicrobial-resistance
dc.subject.keywordsEmerging contaminants
dc.subject.keywordsFluorescence-based detection
dc.subject.keywordsFluorescence microscopy
dc.subject.keywordsSpectrophotometry
dc.titleOptical Detection of Azithromycin in Water and Urine Using Molecularly Imprinted Polymer-Microfluidics Integration
dc.typeElectronic Thesis or Dissertation

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