Exercise Modifies Inflammatory Responses by Changing the Epigenetic Landscape

Simple item page
dc.contributor.advisorAbdul Sater, Ali
dc.contributor.authorAmandeep, Amandeep
dc.date.accessioned2024-03-18T18:04:54Z
dc.date.available2024-03-18T18:04:54Z
dc.date.issued2024-03-16
dc.date.updated2024-03-16T10:36:52Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractPhysical inactivity and low-grade systemic inflammation are associated with chronic metabolic diseases such as atherosclerosis, insulin resistance, obesity etc. It is of great value to study the exercise mediated benefits to alleviate inflammation. Preliminary work in our lab demonstrated that exercise with a particular intensity and duration led to reduced inflammatory responses in bone marrow derived macrophages (BMDMs) by inducing persistent metabolic changes in these cells. In this thesis, we explored how exercise is causing these long-lasting changes in inflammatory responses and hypothesized that it does so by inducing changes in chromatin accessibility leading to changes in gene expression. We employed an unbiased ATAC-seq (Assay for Transposase Accessible Chromatin- sequencing) analysis in BMDMs prepared from sedentary and exercised C57BL/6N mice and obtained a list of differentially accessible chromatin regions. We also demonstrated using real-time PCR that exercise-mediated changes in chromatin accessibility cause a reduction in gene expression of inflammatory genes. Our findings suggested that exercise is protective against inflammatory responses by causing persistent changes at chromatin level.
dc.identifier.urihttps://hdl.handle.net/10315/41919
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectKinesiology
dc.subjectHealth sciences
dc.subject.keywordsExercise
dc.subject.keywordsInflammation
dc.subject.keywordsChromatin accessibility
dc.subject.keywordsTrained Immunity
dc.subject.keywordsImmune system
dc.titleExercise Modifies Inflammatory Responses by Changing the Epigenetic Landscape
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Amandeep_Amandeep_2021_Masters.pdf
Size:
3.73 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.87 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.39 KB
Format:
Plain Text
Description:
Download

Collections

Kinesiology & Health Science

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.