Regulation of Skeletal Muscle Glucose and Fat Metabolism by DHA and EPA

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dc.contributor.advisorCeddia, Rolando
dc.contributor.authorKatsnelson, Glen
dc.date.accessioned2019-11-22T18:47:14Z
dc.date.available2019-11-22T18:47:14Z
dc.date.copyright2019-06
dc.date.issued2019-11-22
dc.date.updated2019-11-22T18:47:14Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThis study investigated whether docosahexaenoic (DHA) and eicosapentaenoic (EPA) acids can directly regulate glucose and fat metabolism in skeletal muscle, besides exerting anti-inflammatory effects. To accomplish this, L6 skeletal muscle cells were treated with 50M of either DHA or EPA for 1, 3, and 5 days. We found that glucose uptake and glycogen synthesis as well as AKT and GSK3 phosphorylation remained unaffected. However, glucose and palmitate oxidation rates were consistently increased (day 1-5) by DHA treatment, whereas EPA increased this variable transiently (day 1). Similarly, only DHA caused significant and sustained increases in AMPK phosphorylation and contents of CPT1b and PGC-1. DHA also had a more potent anti-inflammatory effect than EPA. In conclusion, besides exerting anti-inflammatory effects, EPA, and to a greater extent, DHA, directly regulated glucose and fat metabolism in skeletal muscle cells. Thus, by directly enhancing glucose and fat oxidation, DHA may protect skeletal muscle against lipotoxicity.
dc.identifier.urihttp://hdl.handle.net/10315/36724
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectNutrition
dc.subject.keywordsFat oxidation
dc.subject.keywordsinsulin signalling
dc.subject.keywordsPGC-1α
dc.subject.keywordsCPT1b
dc.subject.keywordsAMPK
dc.subject.keywordsinflammation
dc.subject.keywordsEPA
dc.subject.keywordsDHA
dc.titleRegulation of Skeletal Muscle Glucose and Fat Metabolism by DHA and EPA
dc.typeElectronic Thesis or Dissertation

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