Transcription Factor ZTF-22 Regulates Oxidative Stress Responses in Caenorhabditis Elegans

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dc.contributor.advisorKubiseski, Terrance J.
dc.contributor.authorSaleem, Fozia
dc.date.accessioned2023-08-04T15:04:29Z
dc.date.available2023-08-04T15:04:29Z
dc.date.issued2023-08-04
dc.date.updated2023-08-04T15:04:29Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractOxidative stress is caused when the balance between Reactive Oxygen Species (ROS) and detoxification enzymes is disturbed. In Caenorhabditis elegans (C. elegans), gst-4 is an important detoxification enzyme that is activated by the SKN-1 transcription factor when the cell experiences oxidative stress. Our lab previously showed that SKN-1 is negatively regulated by BRAP-2 and brap-2 mutants show elevated levels of gst-4. Our lab performed RNAi screening and found that the knockdown of another transcription factor, ZTF-22, also enhanced gst-4 levels. Therefore, ZTF-22 was selected for further investigation in the context of oxidative stress. I found that loss of ZTF-22 caused an increased expression of gst-4, and sod-3. I also found that ztf-22 deletion caused lifespan extension, reduced survival under heat stress and increased survival under the ER stress. Therefore, ZTF-22 contributes to the oxidative stress response, but also plays biological roles in thermotolerance, longevity, and the ER stress response.
dc.identifier.urihttps://hdl.handle.net/10315/41289
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subjectMolecular biology
dc.subject.keywordsCaenorhabditis elegans
dc.subject.keywordsC. elegans
dc.subject.keywordsZTF-22
dc.subject.keywordsSKN-1
dc.subject.keywordsgst-4
dc.subject.keywordsbrap-2
dc.subject.keywordsThermotolerance
dc.subject.keywordsER stress
dc.subject.keywordsLongevity
dc.subject.keywordsSodium arsenite
dc.subject.keywordstert-butyl hydroperoxide
dc.subject.keywordstBHP
dc.subject.keywordsLifespan
dc.titleTranscription Factor ZTF-22 Regulates Oxidative Stress Responses in Caenorhabditis Elegans
dc.typeElectronic Thesis or Dissertation

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