The Role of Autophagy in the Maintenance of Mitochondrial Quality and Adaptations to Contractile Activity

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dc.contributor.advisorHood, David
dc.creatorParousis, Alexandra
dc.date.accessioned2015-08-28T13:09:02Z
dc.date.available2015-08-28T13:09:02Z
dc.date.copyright2014-04-23
dc.date.issued2015-08-28
dc.date.updated2015-08-28T13:09:02Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractAutophagy is a critical survival mechanism facilitating protein turnover and pathogen defense in post-mitotic cells. More recently, mitophagy has been identified for the selective recognition and targeting of mitochondria for degradation. Mitochondrial availability is the net result of organelle catabolism via mitophagy and mitochondrial biogenesis. Although the latter process has been well described, mitophagy in skeletal muscle is less understood, and it is unknown how these two opposing mechanisms converge during contractile activity. We employed an in vitro model of chronic contractile activity (CCA) of myotubes in cell culture in the presence of autophagy inhibitor Bafilomycin A1 (BafA) and assessed the implications of defective autophagy on the adaptations to contractile activity. BafA lead to severe deficits to mitochondrial respiration and enhanced reactive oxygen species. CCA ameliorated this defective phenotype, reverting levels back towards untreated controls. Thus, contractile activity normalizes mitophagy flux and maintains a healthier mitochondrial profile during autophagy deregulation.
dc.identifier.urihttp://hdl.handle.net/10315/29797
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectCellular biology
dc.subjectKinesiology
dc.subject.keywordsAutophagy
dc.subject.keywordsContractile activity
dc.subject.keywordsMuscle
dc.subject.keywordsCells
dc.subject.keywordsMitochondria
dc.subject.keywordsBiogenesis
dc.subject.keywordsLC3-II
dc.subject.keywordsPINK1
dc.subject.keywordsParkin
dc.subject.keywordsMitophagy
dc.subject.keywordsSkeletal Muscle
dc.subject.keywordsExercise
dc.subject.keywordsReactive Oxygen Species
dc.subject.keywordsROS
dc.subject.keywordsRespiration
dc.subject.keywordsAntioxidant
dc.subject.keywordsC2C12 cells
dc.titleThe Role of Autophagy in the Maintenance of Mitochondrial Quality and Adaptations to Contractile Activity
dc.typeElectronic Thesis or Dissertationen_US

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