Novel Interaction, Regulation of Phosphorylation and Mitotic Localization of Cell Division Cycle Associated Protein 7 (CDCA7)

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dc.contributor.advisorScheid, Michael P.
dc.creatorParial, Shaon Badhan
dc.date.accessioned2018-08-27T16:41:56Z
dc.date.available2018-08-27T16:41:56Z
dc.date.copyright2018-04-12
dc.date.issued2018-08-27
dc.date.updated2018-08-27T16:41:55Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractCell Division Cycle Associated Protein 7 (CDCA7) is a novel protein with poorly defined physiological significance. The proximity labeling method known as Biotin Identification (BioID) was used to determine candidate interactors of CDCA7. Mass spectrometry identified the microtubule spindle assembly protein TPX2 as a novel candidate interactor of CDCA7. Immunoprecipitation experiments verified interaction between CDCA7 and TPX2. The region of interaction was isolated to residues 195-204 of CDCA7. Based on inhibitor experiments, Aurora A kinase activity coincides with the phosphorylation of CDCA7 at the threonine (T) 163 residue. During mitosis, CDCA7 colocalizes with TPX2, localizes at centrosomes and undergoes phosphorylation at T163. These findings and previous findings in literature place CDCA7, TPX2 and potentially Aurora A kinase together within the context of mitosis. The results here demonstrate the direct interaction between CDCA7 and TPX2, and highlight residue T163 of CDCA7 as a prospective phosphorylation target of Aurora A kinase.
dc.identifier.urihttp://hdl.handle.net/10315/35017
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsMolecular biology
dc.subject.keywordsCellular Biology
dc.subject.keywordsBiochemistry
dc.subject.keywordsCancer
dc.subject.keywordsTumorigenesis
dc.subject.keywordsCell Division Cycle Associated Protein 7
dc.subject.keywordsCDCA7
dc.subject.keywordsTarget Protein for Xenopus centrosomal kinesin-like protein 2
dc.subject.keywordsTPX2
dc.subject.keywordsAurora A Kinase
dc.subject.keywordsTransfection
dc.subject.keywordsWestern Blot
dc.subject.keywordsImmunoprecipitation
dc.subject.keywordsCell cycle synchronization
dc.subject.keywordsFlow cytometry
dc.subject.keywordsMicroscopy
dc.subject.keywordsProximity Dependent Biotin Identification
dc.subject.keywordsBioID
dc.subject.keywordsMass spectrometry
dc.subject.keywordsProtein-protein interaction
dc.subject.keywordsEvolutionarily Conserved DNA sequences
dc.subject.keywordsPhosphorylation
dc.subject.keywordsKinase Inhibitor
dc.subject.keywordsLocalization
dc.subject.keywordsColocalization
dc.subject.keywordsProposed Model
dc.titleNovel Interaction, Regulation of Phosphorylation and Mitotic Localization of Cell Division Cycle Associated Protein 7 (CDCA7)
dc.typeElectronic Thesis or Dissertation

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