Understanding the Molecular Mechanism of Signal Transduction in LiaSR Two Component System in Bacillus Subtilis

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dc.contributor.advisorStutchbury, Bridget J.
dc.creatorJani, Shailee Bhavan
dc.date.accessioned2018-05-28T12:45:26Z
dc.date.available2018-05-28T12:45:26Z
dc.date.copyright2017-10-04
dc.date.issued2018-05-28
dc.date.updated2018-05-28T12:45:26Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe Bacillus subtilis two-component system (TCS) LiaSR responds to environmental stresses inducing cell envelope damage. Here, the signal transduction mechanisms of LiaS/R are investigated in order to comprehend its uniqueness compared to other TCSs. My results indicate that the soluble portion of LiaS autophosphorylates and plays a bifunctional role towards LiaR. LiaR undergoes phosphorylation by acetyl phosphate in a time dependent manner. LiaR and its mutants bind to distinct regions on the liaSR promoter pre and post-phosphorylation. LiaR function is controlled independently by the N and C terminal domains. Characterization of the effector domain mutants created based on the homologous protein from Enterococcus faecalis suggested that the dimerization interface of LiaR lies on the N-terminal domain. Additionally, signal transduction between LiaSR and Staphylococcus aureus VraSR indicated possible in vivo interspecies cross-communication. In conclusion, my research provides comprehensive analysis of the LiaS/R signal transduction pathways regulating cellular responses in B. subtilis.
dc.identifier.urihttp://hdl.handle.net/10315/34483
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsBacteria
dc.subject.keywordsAntibiotic drug resistance
dc.subject.keywordsB.subtilis
dc.subject.keywordsTwo component system
dc.subject.keywordsLiaSR
dc.subject.keywordsHistidine Kinase
dc.subject.keywordsResponse regulator
dc.subject.keywordsPhosphotransfer
dc.subject.keywordsAutophosphorylation
dc.subject.keywordsCross talk
dc.titleUnderstanding the Molecular Mechanism of Signal Transduction in LiaSR Two Component System in Bacillus Subtilis
dc.typeElectronic Thesis or Dissertation

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