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Characterization of a Highly Dynamic Region in Trag from the F Plasmid

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dc.contributor.advisorAudette, Gerald F.
dc.contributor.authorBragagnolo, Nicholas Joseph
dc.date.accessioned2020-05-11T12:47:52Z
dc.date.available2020-05-11T12:47:52Z
dc.date.copyright2019-09
dc.date.issued2020-05-11
dc.date.updated2020-05-11T12:47:52Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe Escherichia coli F plasmid is representative of conjugative type IV secretion system for the transmission of mobile DNA elements in bacteria, a contributor to widespread antibiotic resistance. The TraG protein of this system consists of a membrane-bound N-terminal domain and a periplasmic C-terminal domain, denoted TraG*. TraG* is essential in preventing redundant DNA transfer. In the donor cell it interacts with TraN within the outer membrane to facilitate mating pair stabilisation. However, TraG* also interacts with a cognate TraS in the inner membrane of the recipient cell to prevent conjugation when the recipient cell carries the same plasmid. This thesis presents structural studies of TraG*; Thermofluor, circular dichroism and HDX-MS experiments showed N-terminal truncation mutants displayed higher stability and less disordered content relative to full-length TraG*. The 45 N-terminal residues of TraG* were predicted to be highly dynamic, possibly serving as a flexible linker between two independently functioning domains.
dc.identifier.urihttps://hdl.handle.net/10315/37421
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular chemistry
dc.subject.keywordsBacterial Conjugation
dc.subject.keywordsEntry Exclusion
dc.subject.keywordsTraG
dc.subject.keywordsBacteriology
dc.subject.keywordsStructural Biochemistry
dc.subject.keywordsProtein Intrinsic Disorder
dc.subject.keywordsGram Negative Bacteria
dc.subject.keywordsAntibiotic Resistance
dc.subject.keywordsMass Spectrometry
dc.subject.keywordsCircular Dichroism
dc.subject.keywordsThermofluor
dc.subject.keywordsX-Ray Crystallography
dc.subject.keywordsProtein Crystallography
dc.titleCharacterization of a Highly Dynamic Region in Trag from the F Plasmid
dc.typeElectronic Thesis or Dissertation

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