Transcriptional Regulation of Pokeweed Antiviral Protein

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dc.contributor.advisorHudak, Katalin A.
dc.contributor.authorDiaz, Camille Anne
dc.date.accessioned2020-05-11T12:37:55Z
dc.date.available2020-05-11T12:37:55Z
dc.date.copyright2019-09
dc.date.issued2020-05-11
dc.date.updated2020-05-11T12:37:54Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractPhytolacca americana (American pokeweed) expresses pokeweed antiviral protein (PAP), a ribosome-inactivating protein (RIP) thought to function in defence. Several PAP isoforms are differentially responsive to jasmonic acid (JA), a phytohormone that mediates resistance against pathogen infections and herbivory. However, few RIP promoters have been characterized and little is known about RIP regulation in their native contexts. In this study, I identified the PAP promoters and discovered long introns in the 5 untranslated regions (5UTRs) of PAP genes. qRT-PCR and reporter assays revealed that both the PAP-I promoter and intron can independently drive gene expression, leading to the transcription of mRNA variants with distinct 5UTRs. In addition, a G-BOX promoter element was found to be required for the JA-mediated upregulation of PAP-I. Differential expression analysis confirmed that several PAP isoforms are responsive to other stresses, in addition to JA. This foundational work sheds light on the endogenous regulation of PAP.
dc.identifier.urihttps://hdl.handle.net/10315/37361
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subject.keywordsAlternative promoter
dc.subject.keywordsIntron
dc.subject.keywordsGene regulation
dc.subject.keywordsAntiviral protein
dc.subject.keywordsPokeweed
dc.subject.keywordsPokeweed antiviral protein
dc.subject.keywordsPlant molecular biology
dc.subject.keywordsTranscription
dc.subject.keywordsPlant hormone
dc.subject.keywordsStress response
dc.subject.keywordsJasmonic acid
dc.titleTranscriptional Regulation of Pokeweed Antiviral Protein
dc.typeElectronic Thesis or Dissertation

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