Biomedical Characterization of the WalKR and the LytSR Two Component System From Staphylococcus Aureus

Simple item page
dc.contributor.advisorGolemi-Kotra, Dasantila
dc.creatorPatel, Kevin Hasmukhlal
dc.date.accessioned2015-08-28T14:38:46Z
dc.date.available2015-08-28T14:38:46Z
dc.date.copyright2014-01-17
dc.date.issued2015-08-28
dc.date.updated2015-08-28T14:38:45Z
dc.degree.disciplineChemistry
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractStaphylococcus aureus is a major gram-positive pathogen, because of its remarkable capacity to develop resistance against different antimicrobial agents. It has been shown that in S. aureus two component systems (TCS) play an important role in responding to stress induced by antibiotics. Given the regulatory importance of TCS, herein we investigate in vitro the signal transduction mechanism of the WalKR, and the LytSR TCSs. These TCSs play a role in susceptibility towards cell wall active antibiotics and cationic antimicrobial agents, respectively. We found that both kinases, WalK and LytS, are active in vitro and capable of catalyzing ATP-dependent autophosphorylation and subsequent phosphoryl transfer reactions to their cognate response regulators WalR and LytR. In contrast to LytS, the autokinase activity of WalK is highly dependent on K+ and Ca2+ ions. We found that LytR undergoes phosphorylation by small molecule phosphate donors such as acetyl phosphate. Phosphorylation of LytR leads to dimerization of the N-terminal domain. Furthermore, we show that LytR has the ability to bind to the promoter region of lrgAB in its unphosphorylated state. Taken together, these data provide in vitro proof of phosphorylation mediated signal flow in the WalKR and LytSR TCSs.
dc.identifier.urihttp://hdl.handle.net/10315/29821
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiochemistry
dc.subjectCellular biology
dc.subjectChemistry
dc.subject.keywordsStaphylococcus aureus
dc.subject.keywordsTwo Component Systems
dc.subject.keywordsLytSR
dc.subject.keywordsWalKR
dc.subject.keywordsAutophosphorylation
dc.subject.keywordsPhosphotransfer
dc.subject.keywordsCloning
dc.subject.keywordsExpression
dc.subject.keywordsPurification
dc.subject.keywordsElectromobility Shift Assay
dc.subject.keywordsPhos-tag gel
dc.titleBiomedical Characterization of the WalKR and the LytSR Two Component System From Staphylococcus Aureus
dc.typeElectronic Thesis or Dissertationen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Patel_Kevin_H_2014_Masters.pdf
Size:
3.65 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.83 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.38 KB
Format:
Plain Text
Description:
Download

Collections

Chemistry

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.