Effects of Somatostatin Receptor 2 Inhibition on Glucagon Counterregulation and C-Peptide Levels in Hypoglycemia Conditioned Male Sprague-Dawley Rats

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dc.contributor.advisorRiddell, Michael C.
dc.creatorJahangiriesmaili, Mahsa
dc.date.accessioned2019-03-05T14:49:41Z
dc.date.available2019-03-05T14:49:41Z
dc.date.copyright2018-10-22
dc.date.issued2019-03-05
dc.date.updated2019-03-05T14:49:41Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe counterregulatory hormone glucagon prevents hypoglycemia; however, recurrent hypoglycemia attenuates glucagon counterregulation to subsequent hypoglycemia. This may explain why patients with diabetes are at increased risk for severe hypoglycemic events with time. As somatostatin inhibits glucagon secretion, we hypothesize that a somatostatin receptor type II antagonist (SSTR2a), PRL-2903, improves glucagon responses attenuated by recurrent hypoglycemia. Healthy male Sprague-Dawley rats were made hypoglycemic on three consecutive days (blood glucose 1.7-2.2 mmol/L) via insulin administration (10-, 8- and 5-U/kg of Humulin-R on days 1-3, respectively). On day 4, rats were treated with either SSTR2a or vehicle 1 h prior to the induction of hypoglycemia with 5 U/kg of R-insulin. Glucagon levels during hypoglycemia were 2.5-fold higher compared to vehicle, and time to reach hypoglycemia was 3.2-fold longer with SSTR2a pre-treatment. Also, C-peptide levels were lower with SSTR2a compared to vehicle. Hepatic glycogen levels were also lower with SSTR2a pre-treatment compared to vehicle.
dc.identifier.urihttp://hdl.handle.net/10315/35851
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectEndocrinology
dc.subject.keywordsBlood glucose (BG)
dc.subject.keywordsCounterregulation
dc.subject.keywordsGlucagon
dc.subject.keywordsHypoglycemia
dc.subject.keywordsSomatostatin (SST)
dc.subject.keywordsSSTR2a
dc.subject.keywordsC-peptide
dc.subject.keywordsGlycogen
dc.titleEffects of Somatostatin Receptor 2 Inhibition on Glucagon Counterregulation and C-Peptide Levels in Hypoglycemia Conditioned Male Sprague-Dawley Rats
dc.typeElectronic Thesis or Dissertation

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