Identifying Substrates, Interacting Partners and Cofactors of Pirh2: Characterizing the Pirh2-PKCdelta Interaction

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dc.contributor.advisorBenchimol, Samuel
dc.creatorNuaaman, Mais M.
dc.date.accessioned2014-07-15T20:15:23Z
dc.date.available2014-07-15T20:15:23Z
dc.date.copyright2013-11-27
dc.date.issued2014-07-09
dc.date.updated2014-07-09T16:51:39Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractp53 is a central player in the cellular response to stress, allowing cells to cope in the presence of diverse stress signals including DNA damage and oncogene activation. In response to stress, p53 acts as a transcription factor to regulate the expression of protein-coding and non-coding RNA genes that collectively result in cell cycle arrest, senescence or apoptosis. One such p53-regulated gene encodes the Pirh2 protein, an E3 ubiquitin ligase known to ubiquitinate many substrates including p53, p27/Kip1 cell cycle inhibitor and DNA polymerase η. The objective of this project was to validate a putative interaction between Pirh2 and Protein Kinase Cδ and determine if the latter was a substrate for Pirh2-mediated ubiquitination. While data suggest that Pirh2 protein expression negatively correlates with PKCδ protein levels, it could not be confirmed that Pirh2 mediates ubiquitin-dependent degradation of PKCδ protein.en_US
dc.identifier.urihttp://hdl.handle.net/10315/27653
dc.language.isoenen_US
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biologyen_US
dc.subjectBiologyen_US
dc.subjectCellular biologyen_US
dc.subject.keywordsPKCdeltaen_US
dc.subject.keywordsp53en_US
dc.subject.keywordsp53 target genesen_US
dc.subject.keywordsUbiquitinationen_US
dc.subject.keywordsPKCδen_US
dc.subject.keywordsPirh2en_US
dc.titleIdentifying Substrates, Interacting Partners and Cofactors of Pirh2: Characterizing the Pirh2-PKCdelta Interactionen_US
dc.typeElectronic Thesis or Dissertation

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