The Utility Of Hydrogen Deuterium Exchange Mass Spectrometry For Characterization Of Proteins And Their Interactions.

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dc.contributor.advisorWilson, Derek
dc.contributor.authorWolf, Esther Zvia
dc.date.accessioned2024-11-07T11:04:38Z
dc.date.available2024-11-07T11:04:38Z
dc.date.copyright2024-06-21
dc.date.issued2024-11-07
dc.date.updated2024-11-07T11:04:38Z
dc.degree.disciplineChemistry
dc.degree.levelDoctoral
dc.degree.namePhD - Doctor of Philosophy
dc.description.abstractMass spectrometry (MS) offers immense versatility to the structural biology field. Particularly, Hydrogen Deuterium Exchange MS (HDX-MS) can overcome obstacles encountered by more traditional, higher resolution structural characterization methods. What happens when your protein system is just too big, dynamic, dilute, or poorly soluble? Recent advancements in structural prediction and HDX-MS automation have enabled some of the fastest data turnaround to date. This work follows many different scenarios where HDX-MS and MS have been employed, including protein-peptide interactions of the Bcl-2 family, affinity ranking and prediction of a focused small molecule library targeting WDR5, USP3 binding characterization of a small molecule screen hit, the detection of proteolysis targeting chimera (PROTAC)-induced ternary complex formation, and epitope mapping of antibodies targeting the SARS-CoV-2 Spike protein, all made possible by the collaboration of the Dickinson Lab (University of Chicago), Icosagen (Estonia), the Ingerman-James Lab (University of North Carolina at Chapel Hill), and the Structural Genomics Consortium.   
dc.identifier.urihttps://hdl.handle.net/10315/42419
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subjectAnalytical chemistry
dc.subjectBiochemistry
dc.subject.keywordsMass spectrometry
dc.subject.keywordsHydrogen-deuterium exchange
dc.subject.keywordsProtein
dc.subject.keywordsSmall molecule
dc.subject.keywordsAntibody
dc.subject.keywordsCOVID
dc.subject.keywordsSARS-CoV-2
dc.subject.keywordsFBXO22
dc.subject.keywordsNSD2
dc.subject.keywordsPWWP1
dc.subject.keywordsPROTAC
dc.subject.keywordsUSP3
dc.subject.keywordsDUBTAC
dc.subject.keywordsBcl-2
dc.subject.keywordsMcl-1
dc.subject.keywordsBH3
dc.subject.keywordsBid
dc.subject.keywordsNoxa
dc.subject.keywordsBim
dc.subject.keywordsBad
dc.subject.keywordsStructural biology
dc.subject.keywordsBinding site
dc.subject.keywordsAllostery
dc.subject.keywordsStructural Genomics Consortium
dc.subject.keywordsIcosagen
dc.subject.keywordsEpitope Mapping
dc.subject.keywordsScreening
dc.titleThe Utility Of Hydrogen Deuterium Exchange Mass Spectrometry For Characterization Of Proteins And Their Interactions.
dc.typeElectronic Thesis or Dissertation

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