The Role of a -Catenin - FMRP Complex In Skeletal Muscle

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dc.contributor.advisorMcDermott, John Charles
dc.creatorChimenti, Michael Alfredo
dc.date.accessioned2019-07-02T16:21:54Z
dc.date.available2019-07-02T16:21:54Z
dc.date.copyright2019-04-05
dc.date.issued2019-07-02
dc.date.updated2019-07-02T16:21:54Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractSkeletal muscle consists of multinucleated myofibers which generate contractile force and regulate glucose levels in the human body. Over time, skeletal muscle can become damaged and therefore requires a robust capacity to self-renew. One of the emerging areas of skeletal muscle research involves studying the post-transcriptional mechanisms of gene regulation which mediate this self-renewal pathway. In this study we present the role of the -catenin-FMRP complex in skeletal muscle. First, we confirmed that -catenin and FMRP interacted through biochemical assays and overexpression of -catenin and FMRP fluorescent fusion proteins. Next, we found that knockdown of -catenin in C2C12 myoblasts undergoing the growth phase led to a significant increase in global translational activity. Finally we identified the presence of -catenin at the translational machinery. In conclusion, this study suggests that the -catenin-FMRP complex may serve as a potential pharmacological target in muscle wasting disorders where protein synthesis is greatly impaired.
dc.identifier.urihttp://hdl.handle.net/10315/36313
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subject.keywordsSkeletal muscle
dc.subject.keywordsBiology
dc.subject.keywordsFragile X Mental Retardation Protein
dc.subject.keywordsFMRP
dc.subject.keywordsβ-catenin
dc.subject.keywordsBeta-catenin
dc.subject.keywordstranslation
dc.subject.keywordspost-transcriptional
dc.subject.keywordsmyofiber
dc.subject.keywordsC2C12 myoblast
dc.titleThe Role of a -Catenin - FMRP Complex In Skeletal Muscle
dc.typeElectronic Thesis or Dissertation

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