The Interplay Between Beta-Hydroxybutyrate, Insulin and Glucose on Regulating MCF7 Cell Cycle Status

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dc.contributor.advisorConnor, Michael
dc.contributor.authorTawadrous, Monica
dc.date.accessioned2020-11-13T14:05:09Z
dc.date.available2020-11-13T14:05:09Z
dc.date.copyright2020-10
dc.date.issued2020-11-13
dc.date.updated2020-11-13T14:05:09Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe Ketogenic diet (KD), has been used to treat epileptic seizures, improve insulin sensitivity and reduce adiposity. This study demonstrates an in-vitro approach to stimulate the KD in order to evaluate the effects on MCF7 breast cancer cell-cycle progression. Under the external conditions of decreased blood insulin, decreased circulating glucose and increased beta-hydroxybutyrate (BHB), all factors which accompany consumption of the KD, revealed preliminary evidence displaying the expression of cell cycle regulating proteins responsible for reducing cellular proliferation. Results illustrate that in depleted glucose conditions, expression of Akt signalling pathway is downregulated, which is responsible for cell survival. AMPK, an energy sensing pathway is upregulated in response to BHB supplementation in low glucose states, which stabilizes downstream p27 protein that assists in halting cell cycle progression. Together, these findings provide evidence that creating a negative growth environment for MCF7 cells has the potential to implicate cellular energy and attenuate cell proliferation.
dc.identifier.urihttp://hdl.handle.net/10315/38004
dc.languageEN
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectHealth sciences
dc.subject.keywordsCancer
dc.subject.keywordsBiology
dc.subject.keywordsBreast cancer
dc.subject.keywordsKetogenic diet
dc.subject.keywordsNutrition
dc.subject.keywordsCancer biology
dc.subject.keywordsMetabolism
dc.titleThe Interplay Between Beta-Hydroxybutyrate, Insulin and Glucose on Regulating MCF7 Cell Cycle Status
dc.typeElectronic Thesis or Dissertation

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