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The Role of Myosin Phosphorylation on Bivalent Oscillations in Mesostoma ehrenbergii Primary Spermatocytes

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dc.contributor.advisorForer, Arthur
dc.creatorSilverio, Amanda
dc.date.accessioned2017-07-27T13:57:05Z
dc.date.available2017-07-27T13:57:05Z
dc.date.copyright2017-04-13
dc.date.issued2017-07-27
dc.date.updated2017-07-27T13:57:05Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe main aspect of my thesis looks at the role of myosin phosphorylation and total myosin inhibition on bivalent oscillations during prometaphase in primary spermatocytes of Mesostoma ehrenbergii. Previous studies have shown that myosin is required for proper chromosome movements in a variety of different cell types. I concentrated on increasing and decreasing the activity of myosin through inhibiting different phosphorylation pathways as well as directly inhibiting myosin. I studied the effects of enhancers and inhibitors of myosin activity on chromosome oscillations. The experiments showed that there are redundant pathways for phosphorylation of the myosin involved in spindle force production. My thesis also looks at the effects of myosin phosphorylation on distance segregation of univalents. Pharmacological studies enhancing or inhibiting myosin showed that myosin may be involved in the number of univalent movements during prometaphase, that a single phosphorylation pathway is involved, and that there are additional pathways that contribute to these movements.
dc.identifier.urihttp://hdl.handle.net/10315/33615
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectCellular biology
dc.subject.keywordsMyosin
dc.subject.keywordsPhosphorylation
dc.subject.keywordsMLCK
dc.subject.keywordsROCK
dc.subject.keywordsPP1
dc.subject.keywordsML-7
dc.subject.keywordsH-7
dc.subject.keywordsY-27632
dc.subject.keywordsBDM
dc.subject.keywordsOkadaic acid
dc.subject.keywordsCalyculin A
dc.subject.keywordsMesostoma ehrenbergii
dc.subject.keywordsPrimary spermatocytes
dc.subject.keywordsCell division
dc.subject.keywordsMeiosis
dc.subject.keywordsPrometaphase
dc.subject.keywordsSpindle
dc.subject.keywordsBivalent oscillations
dc.subject.keywordsDistance segregation
dc.subject.keywordsPrecocious cleavage furrow
dc.subject.keywordsSpindle myosin
dc.subject.keywordsOscillation velocity
dc.subject.keywordsOscillation amplitude
dc.subject.keywordsOscillation period
dc.subject.keywordsMyosin inhibition
dc.subject.keywordsMyosin enhancement
dc.subject.keywordsPhosphorylation pathways
dc.titleThe Role of Myosin Phosphorylation on Bivalent Oscillations in Mesostoma ehrenbergii Primary Spermatocytes
dc.typeElectronic Thesis or Dissertation

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