Exploring Gene Expression Strategies in Potato Leafroll Virus

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dc.contributor.advisorWhite, K. Andrew
dc.contributor.authorArrigo, Julia
dc.date.accessioned2024-03-18T18:11:59Z
dc.date.available2024-03-18T18:11:59Z
dc.date.issued2024-03-16
dc.date.updated2024-03-16T10:52:43Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractPotato leafroll virus (PLRV) is a positive-sense single-stranded RNA plant virus in the genus Polerovirus (family Solemoviridae). This virus is known to use many gene expression strategies during viral infection, including subgenomic messenger RNA (sg mRNA) transcription and non-AUG initiation. In addition, it is known to produce a small viral RNA (svRNA) degradation product by cellular exoribonuclease digestion. The goal of this thesis was to investigate PLRV gene expression strategies and corresponding translational products. Two conserved structural elements, a sg mRNA promoter element, and a downstream stem loop (dSL), were identified. A previously defined RNA element involved in generating svRNA was also investigated. The results uncovered a sg mRNA transcriptional promoter element, a potential coding function for svRNA and no role for the dSL in modulating viral protein translation. These findings contribute to understanding how PLRV expresses its viral proteins during infections.
dc.identifier.urihttps://hdl.handle.net/10315/41928
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subjectVirology
dc.subjectPlant biology
dc.subject.keywordsPotato leafroll virus
dc.subject.keywordsTranscription
dc.subject.keywordsSubgenomic messenger RNA
dc.subject.keywordsSmall viral RNA
dc.subject.keywordsTranslation
dc.subject.keywordsp3a
dc.titleExploring Gene Expression Strategies in Potato Leafroll Virus
dc.typeElectronic Thesis or Dissertation

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