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Exploring the Potential of Polyelectrolyte-Aptamer Films For Use in Optical and Electrochemical Sensing

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dc.contributor.advisorChen, Jennifer I-Ling
dc.creatorMalile, Brian
dc.date.accessioned2016-09-20T16:41:51Z
dc.date.available2016-09-20T16:41:51Z
dc.date.copyright2015-12-10
dc.date.issued2016-09-20
dc.date.updated2016-09-20T16:41:50Z
dc.degree.disciplineChemistry
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractPolyelectrolyte-aptamer films have emerged as versatile and easy-to-fabricate materials for applications ranging from cargo delivery to sensing. The films comprising charged polymers and DNA aptamers exhibit changes in the permeability upon binding of the target. Herein we explore the target-controlled diffusion of ions in these films as the basis for generating sensing signals. First, we demonstrate a colorimetric sensing platform, where the polyelectrolyte-aptamer film is assembled on top of plasmonic nanoparticles. The colorimetric response arises from morphological changes of nanoparticles induced by an oxidizing etching agent, whose rate of diffusion is controlled by the polyelectrolyte-aptamer film. Second, we show the versatility of these films for electrochemical sensing by assembling polyelectrolyte-aptamer films on Indium Tin Oxide electrodes. Cyclic voltammetry was employed to monitor the diffusion-controlled electrochemical activity of a redox couple that generates the signal. We examine the concentration dependence and ionic interference effects of these two platforms, and investigate the film composition and aptamer requirements for achieving the sensing functionality. In summary, this project provides fundamental insight on a facile and low cost methodology in optical and electrochemical sensing that may be readily adapted to different analytes. Further development of these films and sensing platforms may allow portable field analysis by using consumer electronics. Our work presents new opportunities in aptamer-based sensing.
dc.identifier.urihttp://hdl.handle.net/10315/32193
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectAnalytical chemistry
dc.subject.keywordsSensing
dc.subject.keywordsPolyeletrolyte multilayers
dc.subject.keywordsAptamer
dc.subject.keywordsAptasensor
dc.subject.keywordsElectrochemistry
dc.subject.keywordsOptical sensing
dc.subject.keywordsNanoparticle sensor
dc.titleExploring the Potential of Polyelectrolyte-Aptamer Films For Use in Optical and Electrochemical Sensing
dc.typeElectronic Thesis or Dissertation

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