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Intestinal Immunolocalization and Insight on the Role of Tachykinin-Related Peptides in the Yellow Fever Mosquito, Aedes aegypti

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dc.contributor.advisorPaluzzi, Jean-Paul
dc.contributor.authorKohandel, Zeynab
dc.date.accessioned2024-03-18T18:02:39Z
dc.date.available2024-03-18T18:02:39Z
dc.date.issued2024-03-16
dc.date.updated2024-03-16T10:42:47Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThis study investigates a family of neuropeptides known as tackykinin-related peptides (TKRPs) in the mosquito Ae. aegypti, which is an insect of medical concern owing to its transmission of several arboviruses. As knowledge on TKRPs is limited in this mosquito, this study aimed to create a tk knockout line using CRISPR/Cas9 and characterize the distribution of TKRP immunoreactivity in the midgut over different physiological conditions including starved, sucrose-fed and blood-fed, and across developmental stages. The results demonstrate that TKRP immunoreactivity in the Ae. aegypti midgut is greatest in adult stage mosquitoes. Further, starvation significantly reduced TKRP immunoreactivity in the midgut compared to sugar fed adult mosquitoes, but no change was observed in relation to blood-feeding by females. Overall, this study established the intestinal distribution of TKRPs in Ae. aegypti and identified functional sgRNAs to disrupt the tk gene so that the physiological role of TKRPs can soon be characterized.
dc.identifier.urihttps://hdl.handle.net/10315/41900
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subjectGenetics
dc.subjectBiology
dc.subject.keywordsBiology
dc.subject.keywordsMolecular biology
dc.subject.keywordsGenetics
dc.subject.keywordsCRISPR/Cas9
dc.subject.keywordsAe. aegypti
dc.subject.keywordsMosquito
dc.subject.keywordsYellow fever
dc.titleIntestinal Immunolocalization and Insight on the Role of Tachykinin-Related Peptides in the Yellow Fever Mosquito, Aedes aegypti
dc.typeElectronic Thesis or Dissertation

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