The Role and Mechanism of Nodal in Regulation of Oocyte Maturation in Zebrafish

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dc.contributor.advisorPeng, Chun
dc.contributor.authorMalik, Ramsha
dc.date.accessioned2022-12-14T16:18:01Z
dc.date.available2022-12-14T16:18:01Z
dc.date.copyright2021-05-20
dc.date.issued2022-12-14
dc.date.updated2022-12-14T16:18:00Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractZebrafish is beneficial to study endocrine and paracrine pathways conserved among vertebrates, such as follicle development and oocyte maturation. Luteinizing hormone (LH) induces maturation inducing hormone (MIH) from the ovary to initiate oocyte maturation. Late vitellogenic (stage IIIb) follicles respond to these hormone signals to undergo oocyte maturation. Nodal, a member of the TGF-β superfamily, plays an important role in embryo development. Stage IIIb follicles subjected to Nodal treatment showed oocyte maturation induced in a dose- and time-dependent manner. In vivo, ndr2+/- knockouts had higher progesterone levels but lower 17a-HP, suggesting ndr2 promotes progesterone to 17a-HP conversion. To determine if Nodal regulates the cAMP pathway, regulation of cAMP responsive element binding proteins (CREB) were examined. Treatment of follicles with Nodal resulted in increased CREB-1a levels in oocytes. This study demonstrates that Nodal promotes zebrafish oocyte maturation, probably by upregulating 17a-HP production in follicular cells and downregulating CREB-5a expression in oocytes.
dc.identifier.urihttp://hdl.handle.net/10315/40594
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subjectDevelopmental biology
dc.subjectMolecular biology
dc.subject.keywordsZebrafish
dc.subject.keywordsNodal
dc.subject.keywordsReproduction
dc.subject.keywordsEndocrinology
dc.subject.keywordsFollicle development
dc.subject.keywordsOocyte maturation
dc.subject.keywordsTransforming growth factor beta
dc.subject.keywordscAMP pathway
dc.titleThe Role and Mechanism of Nodal in Regulation of Oocyte Maturation in Zebrafish
dc.typeElectronic Thesis or Dissertation

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