Isothermal titration calorimetry studies of aptamer-small molecule interactions: practicalities and pitfalls
dc.contributor.author | Slavkovic, Sladjana | |
dc.contributor.author | Johnson, Philip E | |
dc.date.accessioned | 2022-07-08T13:43:13Z | |
dc.date.available | 2022-07-08T13:43:13Z | |
dc.date.issued | 2018-06-12 | |
dc.description.abstract | Isothermal titration calorimetry (ITC) is a powerful technique for studying binding interactions. From a single ITC experiment it is possible to quantify the affinity and thermodynamics of a binding event. Here, we outline an experimental approach for performing ITC experiments with a focus on aptamer-small molecule interactions. We also discuss some common problems that can be encountered and how to resolve these issues. | en_US |
dc.identifier.citation | Aptamers, 2 (2018) 45-51 | en_US |
dc.identifier.issn | 2514-3247 | |
dc.identifier.uri | http://hdl.handle.net/10315/39531 | |
dc.language.iso | en | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Isothermal titration calorimetry studies of aptamer-small molecule interactions: practicalities and pitfalls | en_US |
dc.type | Article | en_US |
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