The Role of p107 in Myogenic Stem Cell DNA Methylation

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dc.contributor.advisorScimè, Anthony
dc.contributor.authorFine, Kyra Rachel
dc.date.accessioned2024-07-18T21:25:11Z
dc.date.available2024-07-18T21:25:11Z
dc.date.copyright2024-04-23
dc.date.issued2024-07-18
dc.date.updated2024-07-18T21:25:10Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractMyogenic stem cells have the potential to either self-renew or differentiate into muscle fibers. These stem cell fate choices are influenced by metabolic changes which affect the epigenetic landscape, including DNA methylation which alters gene expression patterns. Because we have found that a mitochondrial function of p107, a transcriptional co-repressor, is involved in regulating SC metabolism and fate decisions, we investigated whether p107 influences the mitochondrial DNA methylation signature. For this we used a portable long read sequencer MinION (Nanopore), to sequence and compare the mitochondria DNA methylation pattern of wildtype and p107 genetically deleted (p107KO) C2C12 myogenic cell line. We found that p107KO mitochondrial DNA had decreased methylation levels for OXPHOS genes compared to wild type. Thus, suggesting that p107 might suppress ND4 gene expression. We also compared a novel method of mtDNA isolation to three existing methods in order to determine the most efficient approach for sequencing.
dc.identifier.urihttps://hdl.handle.net/10315/42178
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subjectBioinformatics
dc.subjectGenetics
dc.subject.keywordsSequencing
dc.subject.keywordsMethylation
dc.subject.keywordsBioinformatics
dc.subject.keywordsGenetics
dc.subject.keywordsp107
dc.subject.keywordsC2C12
dc.subject.keywordsMinION
dc.subject.keywordsNanopore
dc.subject.keywordsMyoblasts
dc.subject.keywordsStem cells
dc.subject.keywordsMitochondria
dc.subject.keywordsEpigenetics
dc.subject.keywordsMitogenome
dc.subject.keywordsMitoepigenome
dc.subject.keywordsMouse
dc.subject.keywordsMouse genetics
dc.subject.keywordsCell culture
dc.subject.keywordsDNA isolation
dc.subject.keywordsLong read sequencing
dc.subject.keywordsGenomics
dc.subject.keywordsOXPHOS
dc.subject.keywordsOxidative phosphorylation
dc.subject.keywordsMethylome
dc.subject.keywordsDifferential methylation analysis
dc.subject.keywordsTranscription factors
dc.titleThe Role of p107 in Myogenic Stem Cell DNA Methylation
dc.typeElectronic Thesis or Dissertation

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