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Structural and Functional Analyses of the Interaction Between the USP7-NTD and the E2-Conjugating Enzymes, UbE2E2 and UbE2E3

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dc.contributor.advisorSaridakis, Vivian
dc.creatorAshurov, Leila
dc.date.accessioned2015-01-26T15:24:33Z
dc.date.available2015-01-26T15:24:33Z
dc.date.copyright2014-08-27
dc.date.issued2015-01-26
dc.date.updated2015-01-26T15:24:32Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractUbiquitin-specific protease 7 (USP7) is a deubiquitinating enzyme that regulates the turnover of proteins in a cell. To date several interacting partners that are involved in various cellular processes have been identified for USP7. Many of the interacting partners of USP7 contain a P/AxxS motif. We have identified two E2 ubiquitin-conjugating enzymes, UbE2E2 and UbE2E3, which contain the P/AxxS motif. Using a co-immunoprecipitation assay we have shown that these E2 proteins interact with USP7 through their P/AxxS motif. Co-crystal structures of the N-terminal domain of USP7 (USP7-NTD) and the E2 peptides reveal that the interactions are formed between USP7-NTD residues 164DWGF167 and the E2 P/AxxS motifs. It has also been established that USP7 may be involved in regulating the cellular levels of UbE2E2. Overall, our findings suggest USP7, a de-ubiquitinating enzyme, is a regulator of ubiquitin-conjugating enzymes, which are involved in the ubiquitination cascade.
dc.identifier.urihttp://hdl.handle.net/10315/28292
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiochemistry
dc.subjectCellular biology
dc.subjectMolecular biology
dc.subject.keywordsUbE2E3en_US
dc.subject.keywordsUbiquitinen_US
dc.subject.keywordsUbiquitinationen_US
dc.subject.keywordsUbiquitin-conjugating enzymesen_US
dc.subject.keywordsDeubiquitinating enzymesen_US
dc.subject.keywordsUSP7en_US
dc.subject.keywordsUbE2E2en_US
dc.titleStructural and Functional Analyses of the Interaction Between the USP7-NTD and the E2-Conjugating Enzymes, UbE2E2 and UbE2E3
dc.typeElectronic Thesis or Dissertationen_US

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