Ambient Hypoxia Differentially Impacts Thrombospondin-1 and Thrombospondin-4 in Differentiated Murine Myotubes

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dc.contributor.advisorBirot, Olivier
dc.contributor.authorChamoun, Khalil
dc.date.accessioned2022-03-03T13:55:30Z
dc.date.available2022-03-03T13:55:30Z
dc.date.copyright2021-08
dc.date.issued2022-03-03
dc.date.updated2022-03-03T13:55:30Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractSkeletal muscle microvasculature responds to stimuli with great plasticity through angioadaptation which itself is tightly regulated by a balance of pro- and antiangiogenic factors. THBS-1 has been established as the key antiangiogenic factor in skeletal muscle, and its homologue, THBS-4, has recently been identified as a proangiogenic factor in tissue other than skeletal muscle. Hypoxia has been shown to be a principal component of exercise, a potent angiogenic stimulus, yet its impact on THBS-1 and THBS-4 in myotubes has rarely been examined. My project aimed to investigate the impact of hypoxia on THBS-1 and THBS-4 in differentiated murine myotubes. The findings suggest that 1) myotubes are a source of THBS-1, 2) hypoxia greatly reduces THBS-1 and THBS-4 intracellular expression after 24 hours, and 3) hypoxia differentially impacts THBS-1 and THBS-4 in their protein stability and secretion. My research concludes that hypoxia differentially impacts THBS-1 and THBS-4 in myotubes.
dc.identifier.urihttp://hdl.handle.net/10315/39042
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectPhysiology
dc.subject.keywordsMuscle
dc.subject.keywordsSkeletal Muscle
dc.subject.keywordsThrombospondin
dc.subject.keywordsTHBS
dc.subject.keywordsTHBS-1. THBS-4
dc.subject.keywordsTSP
dc.subject.keywordsTSP-1
dc.subject.keywordsTSP-4
dc.subject.keywordsVEGF
dc.subject.keywordsVEGF-A
dc.subject.keywordsExercise
dc.subject.keywordsEndurance
dc.subject.keywordsTraining
dc.subject.keywordsAcute
dc.subject.keywordsRunning
dc.subject.keywordsClimbing
dc.subject.keywordsCycling
dc.subject.keywordsMouse
dc.subject.keywordsMice
dc.subject.keywordsRat
dc.subject.keywordsRats
dc.subject.keywordsRodents
dc.subject.keywordsHuman
dc.subject.keywordsHypoxia
dc.subject.keywordsAltitude
dc.subject.keywordsIn vitro
dc.subject.keywordsCell
dc.subject.keywordsCulture
dc.subject.keywordsProtein
dc.subject.keywordsRNA
dc.subject.keywordsmRNA
dc.subject.keywordsHalf-life
dc.subject.keywordsMedia
dc.subject.keywordsSecreted
dc.subject.keywordsECM
dc.subject.keywordsAngiogenesis
dc.subject.keywordsAngioadaptation
dc.subject.keywordsAngioadaptive
dc.subject.keywordsAngiostatic
dc.subject.keywordsProangiogenic
dc.subject.keywordsAntiangiogenic
dc.subject.keywordsVascular
dc.subject.keywordsBiology
dc.subject.keywordsCapillaries
dc.subject.keywordsMyotubes
dc.subject.keywordsMyofibers
dc.subject.keywordsEndothelial
dc.subject.keywordsTissue
dc.subject.keywordsTranscription
dc.subject.keywordsFactor
dc.subject.keywordsReceptor
dc.subject.keywordsGlycoprotein
dc.subject.keywordsImpact
dc.subject.keywordsThrombospondin-1
dc.subject.keywordsThrombospondin-4
dc.titleAmbient Hypoxia Differentially Impacts Thrombospondin-1 and Thrombospondin-4 in Differentiated Murine Myotubes
dc.typeElectronic Thesis or Dissertation

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