Elastic tethers remain functional during anaphase arrest in partially-lysed crane-fly spermatocytes, and in non-lysed spermatocytes treated with microtubule inhibitors

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dc.contributor.advisorForer, Arthur H.
dc.contributor.authorAdil, Aisha
dc.date.accessioned2024-03-18T17:59:49Z
dc.date.available2024-03-18T17:59:49Z
dc.date.issued2024-03-16
dc.date.updated2024-03-16T10:48:11Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractElastic tethers connect corresponding arms (telomeres) of partner chromosomes during anaphase segregation; they exert anti-poleward (backward) forces on the poleward-moving chromosomes. This thesis examined how arresting anaphase chromosomal segregation affected elastic tether function. Various dilutions of a standard immunofluorescent lysis buffer were used to partially lyse anaphase-I spermatocytes of crane flies. Partial cell lysis deactivated the anaphase spindle apparatus and arrested anaphase chromosomal segregation. Elastic tethers remained functional, and backward tether forces acting on chromosomes were able to cause backward chromosomal movements. Backward-moving chromosomes were attached to their kinetochore microtubules (kMTs) which might have been slowing them down. To test whether detaching chromosomes from their kMTs could enable their faster backward movements, anaphase spermatocytes were treated with various microtubule inhibitors to disassemble kMTs. The inhibitors led to anaphase segregation arrest, and backward chromosomal movements. However, the drugs were unable to disassemble the stable, acetylated kMTs, and allow faster backward movements.
dc.identifier.urihttps://hdl.handle.net/10315/41885
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectCellular biology
dc.subjectMolecular biology
dc.subject.keywordsTethers
dc.subject.keywordsAnaphase chromosomal movement
dc.subject.keywordsAnti-poleward movement
dc.subject.keywordsCrane-fly spermatocytes
dc.subject.keywordsPartial cell lysis
dc.subject.keywordsLysis buffer
dc.subject.keywordsNon-ionic detergent
dc.subject.keywordsMicrotubule inhibitor
dc.subject.keywordsNocodazole
dc.subject.keywordsPodophyllotoxin
dc.subject.keywordsColcemid
dc.titleElastic tethers remain functional during anaphase arrest in partially-lysed crane-fly spermatocytes, and in non-lysed spermatocytes treated with microtubule inhibitors
dc.typeElectronic Thesis or Dissertation

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