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Electrophoresis- Driven Lateral Flow Immunoassay and Aptamer Selection

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dc.contributor.advisorKrylov, Sergey N.
dc.contributor.authorTeclemichael, Eden Tesfagiorgis
dc.date.accessioned2024-03-18T17:58:58Z
dc.date.available2024-03-18T17:58:58Z
dc.date.issued2024-03-16
dc.date.updated2024-03-16T10:51:15Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractElectrophoresis has become an indispensable tool in Biochemistry and Molecular Biology, essential for analyzing proteins and nucleic acids. My work focuses on new bioanalytical applications of electrophoresis: lateral flow immunoassay (LFIA) and the selection of oligonucleotide aptamers. Electrophoretically-driven LFIA (eLFIA) is a new technique aiming to enhance diagnostic sensitivity of LFIA in both antigen and serological tests. While previously applied to Hepatitis B and C, I aimed to extend eLFIA's scope to analyze the SARS-CoV-2 spike protein, demonstrating a 77% reduction in the limit of detection compared to conventional LFIA. Shifting focus to aptamers, I utilized capillary electrophoresis (CE), with the highest partitioning efficiency, to address challenges in aptamer selection. I determined the optimum target concentration and developed bulk affinity assays workflow that quantitatively assesses the progress of selection. Understanding these parameters can significantly influence aptamer selection efficiency and can guide researchers in designing assays and developing novel diagnostic tools.
dc.identifier.urihttps://hdl.handle.net/10315/41875
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subjectAnalytical chemistry
dc.subjectBiochemistry
dc.subject.keywordsLateral flow immunoassay
dc.subject.keywordsCapillary electrophoresis
dc.subject.keywordsAptamer selection
dc.subject.keywordsAptamers
dc.subject.keywordsCovid-19 rapid test
dc.subject.keywordsPoint of care
dc.subject.keywordsBulk affinity in aptamer selection
dc.subject.keywordsTarget concentration in aptamer selection
dc.subject.keywordsSARS CoV-2 detection
dc.subject.keywordsBiomolecular interactions
dc.subject.keywordsOptimization of lateral flow immunoassays
dc.titleElectrophoresis- Driven Lateral Flow Immunoassay and Aptamer Selection
dc.typeElectronic Thesis or Dissertation

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