The Characterization of Taz Protein-Protein Interactions in Myogenic Cells

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dc.contributor.advisorMcDermott, John
dc.contributor.authorMacKeracher, Anastasia Elizabeth
dc.date.accessioned2025-04-10T10:37:00Z
dc.date.available2025-04-10T10:37:00Z
dc.date.copyright2024-08-01
dc.date.issued2025-04-10
dc.date.updated2025-04-10T10:36:59Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe Hippo signaling pathway is known to regulate cell proliferation, differentiation, and apoptosis. Recent studies identified the Hippo signaling target protein TAZ as a repressor of myogenic differentiation. Since TAZ lacks inherent DNA binding ability, its activity is dependent on its protein interactions. Using GFP-nanoTrap affinity purification and LC-MS/MS, we documented TAZ protein interactions in muscle cells, including the pro-myogenic methyltransferase CARM1. Investigation of the TAZ and CARM1 interaction revealed that CARM1 represses TAZ transcriptional activation, promoting TAZ Ser89 phosphorylation and cytoplasmic sequestration. The TAZ/CARM1 interaction was further investigated in embryonic rhabdomyosarcoma (RD) cells, where Hippo signaling increased compared to control C2C12 myoblasts. Further, mass spectrometry results suggest that TAZ is a substrate of CARM1 methylation, with methylation at Arg 77 enhancing TAZ Ser89 phosphorylation. These findings highlight the potential role of CARM1 in regulating TAZ function in myogenic cells.
dc.identifier.urihttps://hdl.handle.net/10315/42706
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subject.keywordsHippo signaling
dc.subject.keywordsTAZ
dc.subject.keywordsProtein interactions
dc.subject.keywordsMyogenic differentiation
dc.subject.keywordsMethylation
dc.titleThe Characterization of Taz Protein-Protein Interactions in Myogenic Cells
dc.typeElectronic Thesis or Dissertation

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