Regulation of Exercise Induced Endothelial Sprout Formation

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dc.contributor.advisorHaas, Tara L.
dc.creatorLiu, Sammy Tsz Kit
dc.date.accessioned2015-08-28T12:59:53Z
dc.date.available2015-08-28T12:59:53Z
dc.date.copyright2014-03-24
dc.date.issued2015-08-28
dc.date.updated2015-08-28T12:59:53Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractCapillary sprouting is known to be guided by Dll4/Notch signaling in mouse retina, while maturation of the endothelium is regulated by Dll1 and Tie2. This study investigates the key molecules involved in endothelial sprouting and maturation, and the gene that orchestrates the expression of these targets in skeletal muscle in response to exercise. In exercised mice, Dll1 and Dll4 proteins were decreased with repeated training. Tie2 mRNA was downregulated with 5 days of exercise. The suppression of these molecules may induce destabilization of the endothelium and allow for sprouting to occur. Moreover, FoxO transcription factors have been shown to be anti-angiogenic and may negatively regulate genes involved in sprouting. Both Dll1 and Dll4 expression were not altered with repeated exercise in mice with endothelial cell directed conditional deletion of FoxO1/3a/4 (FoxO∆). We have provided insight into the mechanisms behind the initiation of capillary growth in skeletal muscle induced by exercise.
dc.identifier.urihttp://hdl.handle.net/10315/29781
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectPhysiology
dc.subjectKinesiology
dc.subjectMolecular biology
dc.subject.keywords3D
dc.subject.keywordsAcute
dc.subject.keywordsAerobic
dc.subject.keywordsAlpha smooth muscle actin
dc.subject.keywordsAngiogenesis
dc.subject.keywordsAngiogenic
dc.subject.keywordsAngiopoietin
dc.subject.keywordsAngiostatic
dc.subject.keywordsBiology
dc.subject.keywordsCapillary
dc.subject.keywordsCapillary-to-fibre
dc.subject.keywordsCell
dc.subject.keywordsChronic
dc.subject.keywordsCre
dc.subject.keywordsCross-section
dc.subject.keywordsCryosection
dc.subject.keywordsCryostat
dc.subject.keywordsDelta/Notch
dc.subject.keywordsDelta-like ligand
dc.subject.keywordsDll1
dc.subject.keywordsDll4
dc.subject.keywordsDll4/Notch
dc.subject.keywordsDNA
dc.subject.keywordsEndostatin
dc.subject.keywordsEndothelial
dc.subject.keywordsCell
dc.subject.keywordsEndothelial cell
dc.subject.keywordsEndurance
dc.subject.keywordsEndurance Training
dc.subject.keywordsExercise
dc.subject.keywordsExplant
dc.subject.keywordsExpression
dc.subject.keywordsFiber
dc.subject.keywordsFibre type
dc.subject.keywordsForkhead
dc.subject.keywordsFormation
dc.subject.keywordsFoxO
dc.subject.keywordsFoxO1
dc.subject.keywordsFoxO3a
dc.subject.keywordsFoxO4
dc.subject.keywordsFVB
dc.subject.keywordsGenes
dc.subject.keywordsHealth
dc.subject.keywordsHIF-1
dc.subject.keywordsHypoxia
dc.subject.keywordsIHC
dc.subject.keywordsImmunofluorescence
dc.subject.keywordsImmunohistochemistry
dc.subject.keywordsImmunostaining
dc.subject.keywordsInterferon
dc.subject.keywordsKnockout
dc.subject.keywordsLectin
dc.subject.keywordsLocalization
dc.subject.keywordsLong term
dc.subject.keywordsLongitudinal-section
dc.subject.keywordsLoxP
dc.subject.keywordsMaturation
dc.subject.keywordsMice
dc.subject.keywordsMMP
dc.subject.keywordsMolecular
dc.subject.keywordsMouse
dc.subject.keywordsmRNA
dc.subject.keywordsMural cells
dc.subject.keywordsMuscle
dc.subject.keywordsMx1
dc.subject.keywordsNG2
dc.subject.keywordsNotch
dc.subject.keywordsNull
dc.subject.keywordsPathway
dc.subject.keywordsPdgfb
dc.subject.keywordsPericytes
dc.subject.keywordsPhysical activity
dc.subject.keywordsPhysiology
dc.subject.keywordsPoly I:C
dc.subject.keywordsPromoter
dc.subject.keywordsProtein
dc.subject.keywordsRatio
dc.subject.keywordsRecombinase
dc.subject.keywordsRecovery
dc.subject.keywordsRegulate
dc.subject.keywordsRepeated
dc.subject.keywordsRNA
dc.subject.keywordsRunning
dc.subject.keywordsScience
dc.subject.keywordsSema3F
dc.subject.keywordsSemaphorin
dc.subject.keywordsShort term
dc.subject.keywordsSignaling
dc.subject.keywordsSkeletal
dc.subject.keywordsSmooth Muscle
dc.subject.keywordsSpecific
dc.subject.keywordsSprouting
dc.subject.keywordsStabilization
dc.subject.keywordsStalk cells
dc.subject.keywordsTie2
dc.subject.keywordsTip cells
dc.subject.keywordsToll-like
dc.subject.keywordsTranscription Factor
dc.subject.keywordsTransgenic
dc.subject.keywordsTreadmill
dc.subject.keywordsTSP-1
dc.subject.keywordsVascular
dc.subject.keywordsVascular Endothelial Growth Factor
dc.subject.keywordsVASH-1
dc.subject.keywordsVasohibin
dc.subject.keywordsVEGF
dc.subject.keywordsVO2
dc.subject.keywordsWave length
dc.subject.keywordsWhole mount
dc.subject.keywordsKinesiology
dc.subject.keywordsMovement
dc.subject.keywordsDestabilization
dc.subject.keywordsEmbryo
dc.subject.keywordsAdult
dc.subject.keywordsTargets
dc.subject.keywordsMolecule
dc.titleRegulation of Exercise Induced Endothelial Sprout Formation
dc.typeElectronic Thesis or Dissertationen_US

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