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Determination of VraR Binding Activity on Promoters of fmtA, murZ, sgtB and pbp2

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dc.contributor.advisorGolemi-Kotra, Dasantila
dc.creatorYang, Zhifeng
dc.date.accessioned2015-01-26T15:08:01Z
dc.date.available2015-01-26T15:08:01Z
dc.date.copyright2014-08-14
dc.date.issued2015-01-26
dc.date.updated2015-01-26T15:08:01Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractVraSR two-component system rapidly senses the cell wall damage by antibiotics and positively modulates a set of genes (VraSR Regulon) to enhance the resistance phenotype in S. aureus. fmtA, murZ, sgtB and pbp2 are members of VraSR Regulon, and they are involved in cell wall peptidoglycan synthesis in response to cell wall inhibitors such as β-lactams. We investigated VraR binding activity on fmtA promoter/its mutants by in vitro and in vivo experiments. We found VraR can bind to two conserved motifs on fmtA promoter in the formation of a dimer and up-regulate the transcription of fmtA under oxacillin conditions. We also found fmtA had two transcription start sites: -157G was responsible for maintenance of a basal level expression, and -195G was induced by oxacillin treatment. We reported VraR/phosphorylated-VraR binding sequences on promoters of murZ, sgtB and pbp2. Putative transcription start sites of murZ, sgtB and pbp2 were also identified.
dc.identifier.urihttp://hdl.handle.net/10315/28267
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordspbp2en_US
dc.subject.keywordsDasantilaen_US
dc.subject.keywordsVraR binding activityen_US
dc.subject.keywordsPromoteren_US
dc.subject.keywordsfmtAen_US
dc.subject.keywordsmurZen_US
dc.subject.keywordssgtBen_US
dc.titleDetermination of VraR Binding Activity on Promoters of fmtA, murZ, sgtB and pbp2
dc.typeElectronic Thesis or Dissertationen_US

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