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Investigating the Structural Dynamics of Therapeutic Proteins using Time resolved Hydrogen deuterium Exchange Mass Spectrometry

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dc.contributor.advisorWilson, Derek
dc.contributor.authorElSabaawy, Marwa Abdalla Abdelmaksoud Mohamed
dc.date.accessioned2023-12-08T14:26:21Z
dc.date.available2023-12-08T14:26:21Z
dc.date.issued2023-12-08
dc.date.updated2023-12-08T14:26:21Z
dc.degree.disciplineChemistry
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractBiopharmaceutical products have drastically grown as essential treatments in clinical settings. Hence, the drug industry is moving towards protein-based therapeutics with increasing demands for parallel advancement in bioanalytical technologies. This thesis centers around displaying the versatility of coupling front-end analytical methods to mass spectrometers. We implement time-resolved electrospray ionization hydrogen-deuterium exchange (TRESI-HDX) and ion mobility spectroscopy (IMS) MS for uncovering new and exciting insights into therapeutic protein dynamics and protein-substrate interactions on millisecond timescale. Using automated continuous flow injection (CFI) has enhanced the analytical throughput of our results. Collectively, the implications for these results widen the fundamental understanding of the studied proteins. Understanding the crosstalk between dynamics and function may help scientists to design new and improved therapeutics.
dc.identifier.urihttps://hdl.handle.net/10315/41625
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectChemistry
dc.subjectBiochemistry
dc.subjectMedicine
dc.subject.keywordsMass spectrometry
dc.subject.keywordsHydrogen deuterium exchange
dc.subject.keywordsHeparin
dc.subject.keywordsPlasminogen activator inhibitor-1
dc.subject.keywordsPAI-1
dc.subject.keywordsProline
dc.subject.keywordsAlanine
dc.subject.keywordsPAS
dc.subject.keywordsAcute lymphoblastic leukemia
dc.subject.keywordsIon mobility
dc.subject.keywordsNano-ESI
dc.subject.keywordsBlood
dc.subject.keywordsBlood disorders
dc.subject.keywordsLatency
dc.subject.keywordsTRESI
dc.subject.keywordsHDX
dc.subject.keywordsFondaparinux
dc.subject.keywordsL-Asparaginase
dc.subject.keywordsHost cell proteins
dc.subject.keywordsChain ejection model
dc.subject.keywordsIonization mechanism
dc.subject.keywordsVitronectin
dc.titleInvestigating the Structural Dynamics of Therapeutic Proteins using Time resolved Hydrogen deuterium Exchange Mass Spectrometry
dc.typeElectronic Thesis or Dissertation

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