The Role of Lipid Mediator Prostaglandin E2 in Early Neuronal Development

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dc.contributor.advisorCrawford, Dorota Anna
dc.creatorDavidson, Jennilee Mary-Ann
dc.date.accessioned2015-12-16T19:23:25Z
dc.date.available2015-12-16T19:23:25Z
dc.date.copyright2015-08-10
dc.date.issued2015-12-16
dc.date.updated2015-12-16T19:23:25Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractLipid mediator prostaglandin E2 (PGE2) plays a pivotal role in early development of the nervous system. Abnormal PGE2 signaling in neurodevelopment has also been implicated in autism. Increased levels of PGE2 have been attributed to maternal infection and the inflammatory response. My in vitro work shows that elevated levels of PGE2 cause an increase in cytosolic and growth cone calcium levels in differentiated neuroectodermal (NE-4C) cells and a dose- and time-dependent effect on neurite extension length. Furthermore, PGE2 induced subcellular localization of the EP4 receptor to the plasma membrane in NE-4C stem cells and growth cones of differentiated NE-4C cells. My in vivo work shows that prenatal exposure to PGE2 results in differential mRNA levels of two important developmental genes Wnt3a and Fosl1 in the mouse brain at various embryonic stages. This furthers our understanding of the functional implications of abnormal PGE2 signaling in the neurodevelopment of autism.
dc.identifier.urihttp://hdl.handle.net/10315/30696
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectNeurosciences
dc.subjectDevelopmental biology
dc.subjectCellular biology
dc.subject.keywordsProstaglandin E2
dc.subject.keywordsPGE2
dc.subject.keywordsDifferentiated neuroectodermal stem cells
dc.subject.keywordsNE-4C
dc.subject.keywordsNeurons
dc.subject.keywordsGrowth cone
dc.subject.keywordsCytosolic calcium
dc.subject.keywordsCalcium fluctuations
dc.subject.keywordsCalcium homeostasis
dc.subject.keywordsNeurite extension
dc.subject.keywordsAutism Spectrum Disorders
dc.subject.keywordsAutism
dc.subject.keywordsEP4 Receptor
dc.subject.keywordsGolgi apparatus
dc.subject.keywordsPlasma membrane
dc.subject.keywordsLipid signaling pathway
dc.subject.keywordsWnt
dc.subject.keywordsFosl1
dc.subject.keywordsWnt3a
dc.subject.keywordsPrenatal PGE2 exposure
dc.subject.keywordsPrenatal
dc.subject.keywordsNeurodevelopment
dc.subject.keywordsDevelopment
dc.subject.keywordsin vitro
dc.subject.keywordsin vivo
dc.subject.keywordsEP receptor signaling
dc.subject.keywordsMouse
dc.subject.keywordsEmbryonic
dc.subject.keywordsNervous system
dc.titleThe Role of Lipid Mediator Prostaglandin E2 in Early Neuronal Development
dc.typeElectronic Thesis or Dissertation

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