Disruption of Branched-Chain Amino Acid Catabolism Impairs Rat Myoblast Survival and Differentiation

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dc.contributor.advisorAdegoke, Olasunkanmi A J
dc.creatorDhanani, Zameer
dc.date.accessioned2016-09-20T18:51:15Z
dc.date.available2016-09-20T18:51:15Z
dc.date.copyright2016-04-20
dc.date.issued2016-09-20
dc.date.updated2016-09-20T18:51:15Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThis study investigates the role of branched-chain amino acid catabolism in rat myoblast differentiation. The branched-chain amino acids (BCAAs), particularly leucine, have been consistently shown to possess anabolic and anti-catabolic effects in regards to skeletal muscle hypertrophy and skeletal muscle differentiation. Metabolites of branched-chain amino acid catabolism have also been shown to induce similar effects, suggesting that production of these metabolites may mediate the effect of BCAA presence. Our study confirms that the leucine metabolite alpha-ketoisocaproate (KIC) can positively regulate rat myoblast differentiation, and ameliorate conditions of leucine deprivation. Furthermore, we demonstrate that impairing the enzymes BCAT2 and BCKD that produce KIC and other BCAA metabolites results in impaired myoblast differentiation and proliferation. Lastly, we show that myoblasts that have impaired BCAA catabolism become apoptotic and programmed for cell death. Conclusively, our findings demonstrate that BCAA catabolism is an essential process that facilitates myoblast survival and differentiation.
dc.identifier.urihttp://hdl.handle.net/10315/32308
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectHealth sciences
dc.subject.keywordsBCAA
dc.subject.keywordsBranched-chain amino acids
dc.subject.keywordsBCAT2
dc.subject.keywordsBCAT
dc.subject.keywordsBCKD
dc.subject.keywordsBCKDC
dc.subject.keywordsAmino acids
dc.subject.keywordsZameer
dc.subject.keywordsDhanani
dc.subject.keywordsadegoke
dc.subject.keywordsMetabolism
dc.subject.keywordsCatabolism
dc.titleDisruption of Branched-Chain Amino Acid Catabolism Impairs Rat Myoblast Survival and Differentiation
dc.typeElectronic Thesis or Dissertation

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